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Chemical Compound Review:

ACMA 6-chloro-2-methoxy-acridin-9- amine

Synonyms: CHEMBL76954, AG-F-22852, A5806_SIGMA, NSC-15300, NSC15300, ...

Deléage, G. et al., Mohapatra, P.K. et al., Engelbrecht, S. et al., Furrer, E.M. et al., Pringle, M.J. et al., et al.

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Disease relevance of 5-22-12-00225 (Beilstein Handbook Reference)

Fluorescence measurements using the pH sensitive dye 9-amino-6-chloro-2-methoxyacridine in everted membrane vesicles of complemented E. coli EP432 showed high Li(+)/H(+) exchange activity at pH 6, but marginal Na(+)/H(+) antiport activity [1].

High impact information on 5-22-12-00225 (Beilstein Handbook Reference)

Effects of mutations on stability of Vph1p, assembly of the V-ATPase complex, 9-amino-6-chloro-2-methoxyacridine quenching (as a measure of proton transport), and ATPase activity were assessed [2].
The passive proton conductance in UF0 proteoliposomes was measured by time-resolved quenching of 9-amino-6-chloro-2-methoxyacridine or 9-aminoacridine fluorescence following a valinomycin-induced K(+)-diffusion potential [3].
Active proton uptake in lipid vesicles reconstituted with the purified yeast plasma membrane ATPase. Fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine [4].
ATP-dependent fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine proved that the ATPase hybrid was able to pump protons in the absence of Na+ [5].
The efficiency or reconstitution was measured in thylakoids via the rate of phenazinemethosulfate-mediated cyclic photophosphorylation and in E. coli everted vesicles via the degree of 9-amino-6-chloro-2-methoxyacridine fluorescence quenching [6].

Biological context of 5-22-12-00225 (Beilstein Handbook Reference)

Some improvements in procedures for the estimation of rates of both ATP hydrolysis and ATP-dependent 9-amino-6-chloro-2-methoxyacridine (ACMA) fluorescence quenching are described [7].

Anatomical context of 5-22-12-00225 (Beilstein Handbook Reference)

Using fluorescence quenching of 9-amino-6-chloro-2- methoxyacridine induced either by ATP hydrolysis in the ATPase-ATP synthase complex or by succinate oxidation in inverted submitochondrial particles, correlation have been established between ATP hydrolysis, ATP synthesis and the generation and utilization of delta pH [8].

Associations of 5-22-12-00225 (Beilstein Handbook Reference) with other chemical compounds

The organophosphorus (OP) insecticides dimethoate and chlorfenvinphos, at all selected concentrations (0-500 micromol liter-1), reduced the rate of accumulation of uranine and enhanced fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine (ACMA) [9].
No pH gradient is detectable by quenching of 9-amino-6-chloro-2-methoxyacridine; and nigericin is without effect on the oxonol response [10].

References

Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus. Furrer, E.M., Ronchetti, M.F., Verrey, F., Pos, K.M. FEBS Lett. (2007) [Pubmed]
Function of the COOH-terminal domain of Vph1p in activity and assembly of the yeast V-ATPase. Leng, X.H., Manolson, M.F., Forgac, M. J. Biol. Chem. (1998) [Pubmed]
ATP synthase complex from bovine heart mitochondria. Passive H+ conduction through F0 does not require oligomycin sensitivity-conferring protein. Pringle, M.J., Kenneally, M.K., Joshi, S. J. Biol. Chem. (1990) [Pubmed]
Active proton uptake in lipid vesicles reconstituted with the purified yeast plasma membrane ATPase. Fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine. Dufour, J.P., Goffeau, A., Tsong, T.Y. J. Biol. Chem. (1982) [Pubmed]
Construction, expression and characterization of a plasmid-encoded Na(+)-specific ATPase hybrid consisting of Propionigenium modestum F0-ATPase and Escherichia coli F1-ATPase. Kaim, G., Dimroth, P. Eur. J. Biochem. (1994) [Pubmed]
Cross-reconstitution of the F0F1-ATP synthases of chloroplasts and Escherichia coli with special emphasis on subunit delta. Engelbrecht, S., Deckers-Hebestreit, G., Altendorf, K., Junge, W. Eur. J. Biochem. (1989) [Pubmed]
Ultrafast purification and reconstitution of His-tagged cysteine-less Escherichia coli F1Fo ATP synthase. Ishmukhametov, R.R., Galkin, M.A., Vik, S.B. Biochim. Biophys. Acta (2005) [Pubmed]
Correlations between ATP hydrolysis, ATP synthesis, generation and utilization of delta pH in mitochondrial ATPase-ATP synthase. Deléage, G., Penin, F., Godinot, C., Gautheron, D.C. Biochim. Biophys. Acta (1983) [Pubmed]
Effect of dimethoate and chlorfenvinphos on plasma membrane integrity of Synechocystis sp. PCC 6803. Mohapatra, P.K., Schiewer, U. Ecotoxicol. Environ. Saf. (1998) [Pubmed]
ATP-dependent spectral response of oxonol VI in an ATP-Pi exchange complex. Kiehl, R., Hanstein, W.G. Biochim. Biophys. Acta (1984) [Pubmed]

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