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Chemical Compound Review

AG-J-88595     8-[[5-(4-amino-2-oxo- pyrimidin-1-yl)-3,4...

Synonyms: CTK7D9931, AC1L18YS
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Disease relevance of CMP-KDO

  • Using [4'-32P]lipid IVA as the probe, we now demonstrate the existence of cytoplasmic KDO-transferases in Escherichia coli capable of attaching 2 KDO residues, derived from CMP-KDO, to lipid IVA [1].
  • These data define the transcriptional unit of kds B and its translation product in molecular terms, information prerequisite to our understanding of both the mechanism of CMP-KDO formation and the regulation of the KDO metabolic pathway in Gram-negative bacteria [2].
  • Identification, characterization, and developmental regulation of Chlamydia trachomatis 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase [3].
  • In Salmonella typhimurium, where the lipid A species are well characterised, it was previously demonstrated that the addition of a compound which inhibits the enzyme 3-deoxy-manno-octulosonate cytidylytransferase (CMP-KDO synthetase; EC leads to rapid accumulation of lipid A derivatives [4].
  • Lipopolysaccharide (LPS) synthesis was inhibited, new lipid A metabolites accumulated, and growth ceased, when the plant pathogen Agrobacterium tumefaciens and the fish pathogen Aeromonas salmonicida were treated with an antibacterial agent which specifically inhibits CTP:CMP-3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthase) [5].

High impact information on CMP-KDO

  • CMP-KDO synthetase: a plant gene borrowed from gram-negative eubacteria [6].
  • KDO is incorporated into these polymers as CMP-KDO, which is produced in an unusual activation step catalyzed by the enzyme CMP-KDO synthetase [7].
  • The ability of the intact operon to complement CTP synthetase and CMP-KDO deficiencies in mutant Escherichia coli strains indicates that both enzymes are efficiently translated from a single messenger RNA [8].
  • Hydrolysis of CMP-KDO also was found to be subject to a substantial solvent isotope effect (kH/kD = 2.7), which is significantly different from the reported solvent isotope effect for the hydrolysis of sialyglycosides (kH/kD = 0.86) and dependent on both buffer and magnesium ion concentrations [9].
  • Optimum KDO incorporation occurred between pH 8 and 9 and required CTP, prior lipid A precursor accumulation, and a functional kdsB gene product, CMP-KDO synthetase [10].

Biological context of CMP-KDO

  • Inhibition of LPS synthesis by CMP-KDO synthase inhibitor had no effect on the initial kinetics of A. tumefaciens attachment to cultured carrot cells, but did inhibit cell aggregation normally induced by bacterial cellulose synthesis [5].

Associations of CMP-KDO with other chemical compounds


Gene context of CMP-KDO

  • The enzyme 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase; CKS) catalyzes the activation of 3-deoxy-manno-octulosonate (KDO) by forming CMP-KDO [12].


  1. Biosynthesis of lipopolysaccharide in Escherichia coli. Cytoplasmic enzymes that attach 3-deoxy-D-manno-octulosonic acid to lipid A. Brozek, K.A., Hosaka, K., Robertson, A.D., Raetz, C.R. J. Biol. Chem. (1989) [Pubmed]
  2. Primary structure of CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) from Escherichia coli. Goldman, R.C., Bolling, T.J., Kohlbrenner, W.E., Kim, Y., Fox, J.L. J. Biol. Chem. (1986) [Pubmed]
  3. Identification, characterization, and developmental regulation of Chlamydia trachomatis 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase. Wylie, J.L., Iliffe, E.R., Wang, L.L., McClarty, G. Infect. Immun. (1997) [Pubmed]
  4. Accumulation of incomplete metabolic side products of lipid A in Salmonella typhimurium during inhibition of 3-deoxy-D-manno-octulosonate incorporation by a new class of antibacterial agents. Kadam, S.K., Doran, C.C., Goldman, R.C. Can. J. Microbiol. (1989) [Pubmed]
  5. Inhibition of lipopolysaccharide synthesis in Agrobacterium tumefaciens and Aeromonas salmonicida. Goldman, R.C., Capobianco, J.O., Doran, C.C., Matthysse, A.G. J. Gen. Microbiol. (1992) [Pubmed]
  6. CMP-KDO synthetase: a plant gene borrowed from gram-negative eubacteria. Royo, J., Gímez, E., Hueros, G. Trends Genet. (2000) [Pubmed]
  7. A maize homologue of the bacterial CMP-3-deoxy-D-manno-2-octulosonate (KDO) synthetases. Similar pathways operate in plants and bacteria for the activation of KDO prior to its incorporation into outer cellular envelopes. Royo, J., Gómez, E., Hueros, G. J. Biol. Chem. (2000) [Pubmed]
  8. Chlamydia trachomatis CTP synthetase: molecular characterization and developmental regulation of expression. Wylie, J.L., Berry, J.D., McClarty, G. Mol. Microbiol. (1996) [Pubmed]
  9. Why is CMP-ketodeoxyoctonate highly unstable? Lin, C.H., Murray, B.W., Ollmann, I.R., Wong, C.H. Biochemistry (1997) [Pubmed]
  10. Inhibition of exogenous 3-deoxy-D-manno-octulosonate incorporation into lipid A precursor of toluene-treated Salmonella typhimurium cells. Capobianco, J.O., Darveau, R.P., Goldman, R.C., Lartey, P.A., Pernet, A.G. J. Bacteriol. (1987) [Pubmed]
  11. CMP-KDO synthetase: overproduction and application to the synthesis of CMP-KDO and analogs. Sugai, T., Lin, C.H., Shen, G.J., Wong, C.H. Bioorg. Med. Chem. (1995) [Pubmed]
  12. Crystallization and preliminary X-ray crystallographic studies of 3-deoxy-manno-octulosonate cytidylyltransferase from Haemophilus influenzae. Ku, M.J., Yoon, H.J., Ahn, H.J., Kim, H.W., Baek, S.H., Suh, S.W. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
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