Disease relevance of carbamoylcarbamoylformic acid

• Streptococcus faecalis ATCC 11700 uses oxalurate as a sole energy source for growth [1].

High impact information on carbamoylcarbamoylformic acid

• Oxalurate induction of multiple URA3 transcripts in Saccharomyces cerevisiae [2].
• Roles of the Dal82p domains in allophanate/oxalurate-dependent gene expression in Saccharomyces cerevisiae [3].
• The structure was confirmed by the chemical transformation of the dehydro-guanidinohydantoin derivative into a linear oxaluric acid derivative upon hydrolysis at ambient temperature [4].
• Interestingly, DNA repair experiments carried out with formamidopyrimidine DNA N-glycosylase (Fpg) and endonuclease III (endo III) show that oxaluric acid is a substrate for both enzymes [5].
• Like other allantoin pathway genes, oxalurate-mediated induction of allantoin transport required a functional DAL81 gene product [6].

Biological context of carbamoylcarbamoylformic acid

• The first mode of uptake is via an active transport system which: (i) has an apparent Km value of 14 muM, (ii) is absolutely dependent upon energy metabolism, (iii) requires pre-growth of the cultures in the presence of oxaluric acid, gratuitous inducer of the allantoin degradative enzymes, and (iv) is sensitive to nitrogen repression [7].

Associations of carbamoylcarbamoylformic acid with other chemical compounds

• An oxamate carbamoyltransferase and a carbamate kinase, both induced by oxalurate, are involved in this process [1].

Gene context of carbamoylcarbamoylformic acid

• Induction is probably a transcriptionally regulated process, because addition of hydantoin acetate or oxalurate to the culture medium increased the steady-state levels of mRNA encoded by a gene required for allantoin transport (DAL4) [6].
• The latter observations suggest that failure of oxalurate to bring about induction of allophanate hydrolase in cultures growing under repressive conditions does not result from inducer exclusion, but rather from repression of dur1,2 gene expression [8].
• Control of enzyme synthesis in the oxalurate catabolic pathway of Streptococcus faecalis ATCC 11700: evidence for the existence of a third carbamate kinase [1].

Analytical, diagnostic and therapeutic context of carbamoylcarbamoylformic acid

• Isotypic pseudooctahedral complexes of Co, Ni, and Cu with two chelating oxalurate ligands and two water molecules, trans-[M(oxalurate)(2)(H(2)O)(2)], have been synthesized and isolated by a novel progressive crystallization technique [9].

References