Disease relevance of DAL82

• Using an E. coli expression system, we showed that DAL82 protein specifically bound to wild type but not mutant DAL UIS sequences [1].

High impact information on DAL82

• Dal81p is required for inducer responsiveness of LexAp-Dal82p and LexAp coiled-coil(DAL82)-mediated transcription but isn't needed for inducer-dependent activation mediated by a Dal82p containing deletions in both the coiled-coil(DAL82), UIS(ALL)-binding domains [2].
• Gln3p binds to and Gat1p is proposed to bind to single GATA sequences; Dal80p binds to pairs of specifically oriented and spaced GATA sequences, and Dal82p binds to a pathway-specific element, UIS(ALL) [2].
• Roles of the Dal82p domains in allophanate/oxalurate-dependent gene expression in Saccharomyces cerevisiae [2].
• LexA-tethering experiments demonstrated that Dal82p is capable of mediating transcriptional activation [3].
• Here we show that Dal82p is localized to the nucleus and parallels movement of the DNA through the cell cycle [3].

Biological context of DAL82

• DAL82 gene expression did not respond to induction by the allantoin pathway inducer or to nitrogen catabolite repression [4].
• From the nucleotide sequence of the DAL82 gene, we deduced that it encodes a protein with a mass of 29,079 Da that may possess the structural motifs expected of a regulatory protein [4].
• We demonstrated that the DAL81 and DAL82 loci are distinct, unlinked genes situated on chromosomes IX and XIV [4].
• Since the DAL81 product was not an allantoin pathway-specific regulatory factor, the DAL82 product was considered as the more likely candidate to be the DAL UIS binding protein [1].
• The similar phenotypes generated by dal81 and dal82 mutations prompted the question of whether they were allelic [4].

Associations of DAL82 with chemical compounds

• DAL82, a second gene required for induction of allantoin system gene transcription in Saccharomyces cerevisiae [4].
• Previous studies have shown that (i) Dal81p and Dal82p are required for allophanate-induced gene expression in Saccharomyces cerevisiae; (ii) the cis-acting element mediating the induced transcriptional response to allophanate is a dodecanucleotide, UIS(ALL); and (iii) Dal82p binds specifically to UIS(ALL) [3].

Other interactions of DAL82

• High-level expression is inducer dependent, requiring functional DAL81 and DAL82 genes [5].

Analytical, diagnostic and therapeutic context of DAL82

• Electrophoretic mobility shift assays identified a peptide (consisting of Dal82p amino acids 1-85) that is sufficient to bind a DNA fragment containing UIS(ALL) [3].

References