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Gene Review

DAL82  -  Dal82p

Saccharomyces cerevisiae S288c

Synonyms: DURM, N0360, Protein DAL82, YNL314W
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Disease relevance of DAL82

  • Using an E. coli expression system, we showed that DAL82 protein specifically bound to wild type but not mutant DAL UIS sequences [1].

High impact information on DAL82

  • Dal81p is required for inducer responsiveness of LexAp-Dal82p and LexAp coiled-coil(DAL82)-mediated transcription but isn't needed for inducer-dependent activation mediated by a Dal82p containing deletions in both the coiled-coil(DAL82), UIS(ALL)-binding domains [2].
  • Gln3p binds to and Gat1p is proposed to bind to single GATA sequences; Dal80p binds to pairs of specifically oriented and spaced GATA sequences, and Dal82p binds to a pathway-specific element, UIS(ALL) [2].
  • Roles of the Dal82p domains in allophanate/oxalurate-dependent gene expression in Saccharomyces cerevisiae [2].
  • LexA-tethering experiments demonstrated that Dal82p is capable of mediating transcriptional activation [3].
  • Here we show that Dal82p is localized to the nucleus and parallels movement of the DNA through the cell cycle [3].

Biological context of DAL82

  • DAL82 gene expression did not respond to induction by the allantoin pathway inducer or to nitrogen catabolite repression [4].
  • From the nucleotide sequence of the DAL82 gene, we deduced that it encodes a protein with a mass of 29,079 Da that may possess the structural motifs expected of a regulatory protein [4].
  • We demonstrated that the DAL81 and DAL82 loci are distinct, unlinked genes situated on chromosomes IX and XIV [4].
  • Since the DAL81 product was not an allantoin pathway-specific regulatory factor, the DAL82 product was considered as the more likely candidate to be the DAL UIS binding protein [1].
  • The similar phenotypes generated by dal81 and dal82 mutations prompted the question of whether they were allelic [4].

Associations of DAL82 with chemical compounds


Other interactions of DAL82

  • High-level expression is inducer dependent, requiring functional DAL81 and DAL82 genes [5].

Analytical, diagnostic and therapeutic context of DAL82

  • Electrophoretic mobility shift assays identified a peptide (consisting of Dal82p amino acids 1-85) that is sufficient to bind a DNA fragment containing UIS(ALL) [3].


  1. The DAL82 protein of Saccharomyces cerevisiae binds to the DAL upstream induction sequence (UIS). Dorrington, R.A., Cooper, T.G. Nucleic Acids Res. (1993) [Pubmed]
  2. Roles of the Dal82p domains in allophanate/oxalurate-dependent gene expression in Saccharomyces cerevisiae. Scott, S., Abul-Hamd, A.T., Cooper, T.G. J. Biol. Chem. (2000) [Pubmed]
  3. Functional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae. Scott, S., Dorrington, R., Svetlov, V., Beeser, A.E., Distler, M., Cooper, T.G. J. Biol. Chem. (2000) [Pubmed]
  4. DAL82, a second gene required for induction of allantoin system gene transcription in Saccharomyces cerevisiae. Olive, M.G., Daugherty, J.R., Cooper, T.G. J. Bacteriol. (1991) [Pubmed]
  5. Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. ElBerry, H.M., Majumdar, M.L., Cunningham, T.S., Sumrada, R.A., Cooper, T.G. J. Bacteriol. (1993) [Pubmed]
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