High impact information on 21H-Biline-8,12-dipropanoic acid, 18-ethenyl-3-ethyl-1,4,5,15,16,19,22,24-octahydro-2,7,13,17-tetramethyl-1,19-dioxo-, (4R,16R)-

• In this quantitative assay, urinary urobilinogen is oxidized to urobilin with iodate in an acid medium, the pH is adjusted to 6 with sodium acetate, and the mixture is reacted with alcoholic HgCl2 solution, extracted with CHCl3, the measured spectrophotometrically at 513 nm [1].
• METHODS: The MACs were assessed by determining the concentrations of eight short-chain fatty acids (SCFA), mucin, urobilin, b-aspartylglycine, coprastanol and faecal tryptic activity (FTA) [2].
• Urobilin is excreted in both the urine and feces from many mammals, particularly humans [3].
• The suitable buffer solution for extraction of urobilin consisted of 0.1 M Tris-HCl buffer (pH 10.0) containing 0.1 m sodium chloride [4].
• Bilirubin and urobilin, metabolic derivatives of BV, showed slight inhibitory effects on virus replication in the cells [5].

Associations of 21H-Biline-8,12-dipropanoic acid, 18-ethenyl-3-ethyl-1,4,5,15,16,19,22,24-octahydro-2,7,13,17-tetramethyl-1,19-dioxo-, (4R,16R)- with other chemical compounds

• The present procedure modifies the latter assay to obviate described problems as it is based on direct spectrophotometry (or spectrofluorometry) of a zinc complex of urobilin in dimethylsulfoxide [6].
• The microflora also influence the degradation of mucin, the conversion of urobilin to urobilinogen, of cholesterol to coprostanol, and the production of short chain fatty acids [7].

References