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Chemical Compound Review:

Biotin-cmha N'-(2-aminooxyethanoyl)-5- [(1S,2S,5S)-7...

Synonyms: ZINC05192440, AC1L36FY, 139585-03-8, Aldehyde reactive probe, O-(Biotinylcarbazoylmethyl)hydroxylamine

Kubo, K. et al., Atamna, H. et al., Ide, H. et al., Asaeda, A. et al., Nakamura, J. et al.

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Disease relevance of Aldehyde reactive probe

With heat/acid-depurinated calf thymus or bacteriophage f1 DNA, ARP detected femtomoles of AP sites in DNA [1].
Furthermore, ARP sites were detectable in DNA isolated from heat-inactivated X-irradiated (10 Gy) and methyl methanesulfonate (MMS)-treated (5 microM) Escherichia coli cells [1].

High impact information on Aldehyde reactive probe

ARP penetrates the plasma membrane of cells and reacts with AP sites in DNA to form a stable ARP-DNA adduct [2].
A biotin-containing aldehyde-reactive probe (ARP) [Kubo, K., Ide, H., Wallace, S. S. & Kow, Y. W. (1992) Biochemistry 31, 3703-3708] is used to measure AP sites in living cells [2].
By using this ARP-slot-blot assay, the rate of spontaneous depurination of calf thymus DNA was determined [3].
In order to elucidate the specificity of ARP to DNA damages, ARP was incubated with a variety of damaged bases or nucleosides and the reaction mixtures were analyzed by HPLC [4].
The rate of production of ARP sites was proportional to the X-ray dose as well as to the concentration of MMS [1].

Biological context of Aldehyde reactive probe

Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP) [5].

References

A novel, sensitive, and specific assay for abasic sites, the most commonly produced DNA lesion. Kubo, K., Ide, H., Wallace, S.S., Kow, Y.W. Biochemistry (1992) [Pubmed]
A method for detecting abasic sites in living cells: age-dependent changes in base excision repair. Atamna, H., Cheung, I., Ames, B.N. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Nakamura, J., Walker, V.E., Upton, P.B., Chiang, S.Y., Kow, Y.W., Swenberg, J.A. Cancer Res. (1998) [Pubmed]
Synthesis and damage specificity of a novel probe for the detection of abasic sites in DNA. Ide, H., Akamatsu, K., Kimura, Y., Michiue, K., Makino, K., Asaeda, A., Takamori, Y., Kubo, K. Biochemistry (1993) [Pubmed]
Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP). Asaeda, A., Ide, H., Tano, K., Takamori, Y., Kubo, K. Nucleosides Nucleotides (1998) [Pubmed]

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