High impact information on PIK3AP1

• Dof is the product of a fast-evolving gene whose vertebrate homologs, BCAP and BANK, are involved in signaling downstream of the B-cell receptor [1].
• Dual phosphorylation of phosphoinositide 3-kinase adaptor Grb2-associated binder 2 is responsible for superoxide formation synergistically stimulated by Fc gamma and formyl-methionyl-leucyl-phenylalanine receptors in differentiated THP-1 cells [2].
• In cultured cells, Abi-1 promoted phosphorylation of BCAP not only by c-Abl but also by v-Abl [3].
• Binding experiments revealed that the SH3 domain of Abi-1 and the C-terminal polyproline structure of BCAP are involved in interactions between the two [3].
• By screening a human leukocyte cDNA library, we identified BCAP (B-cell adaptor for phosphoinositide 3-kinase) as another Abi-1-interacting protein [3].
• Pik3ap1 (or BCAP) is a TIR-domain containing TLR adapter [4].

Biological context of PIK3AP1

• The phosphorylation sites of BCAP by c-Abl were mapped to five tyrosine residues in the C-terminal region that are well conserved in mammals [3].
• Macrophages deficient in BCAP are hyper-responsive to TLR ligands [4] [5].
• BCAP deficient mice are more susceptible to a model of colitis [4].

References