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Gene Review

traR  -  transcriptional regulator TraR

Agrobacterium tumefaciens

 
 
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Disease relevance of traR

 

High impact information on traR

  • The presence of a plasmid with cloned island traR traI2 genes resulted in excision of ICEMlSym(R7A) in all cells regardless of culture density, indicating that excision may be similarly regulated [2].
  • Although altered by deletions, arc(Chry5) is related to the five-gene arc operon that controls the expression of traR on pTiC58 [4].
  • The alterations in the opines, in turn, necessitated a co-evolutionary change in the opine recognition systems responsible for controlling expression of the traR genes on these two types of Ti plasmids [4].
  • We have discovered a second traR-like gene, trlR, on the octopine-mannityl opine-type Ti plasmids pTi15955 and pTiR10 [5].
  • We show that the traR/traM locus is part of a quorum-sensing system unique to AK631 and propose that this locus is involved in regulating conjugal plasmid transfer [6].
 

Chemical compound and disease context of traR

  • A simple, rapid, sensitive microtiter plate method detecting N-acyl homoserine lactone (HSL)-related compounds was established using an Agrobacterium tumefaciens strain harboring a traG::lacZ/traR reporter gene responsive to HSLs [7].
 

Biological context of traR

  • Localization of OccR-activated and TraR-activated promoters that express two ABC-type permeases and the traR gene of Ti plasmid pTiR10 [1].
  • Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer [5].
 

Associations of traR with chemical compounds

 

Other interactions of traR

  • TraR activated target genes in the presence of AAI and also activated traR and traI themselves, creating two positive-feedback loops [8].
  • The expression of tra, but not the growth rate, was partially restored by increasing the copy number of traR or by disrupting traM, a Ti plasmid gene coding for an antiactivator specific for TraR [9].
 

Analytical, diagnostic and therapeutic context of traR

References

  1. Localization of OccR-activated and TraR-activated promoters that express two ABC-type permeases and the traR gene of Ti plasmid pTiR10. Fuqua, C., Winans, S.C. Mol. Microbiol. (1996) [Pubmed]
  2. Excision and transfer of the Mesorhizobium loti R7A symbiosis island requires an integrase IntS, a novel recombination directionality factor RdfS, and a putative relaxase RlxS. Ramsay, J.P., Sullivan, J.T., Stuart, G.S., Lamont, I.L., Ronson, C.W. Mol. Microbiol. (2006) [Pubmed]
  3. Natural instability of Agrobacterium vitis Ti plasmid due to unusual duplication of a 2.3-kb DNA fragment. Fournier, P., de Ruffray, P., Otten, L. Mol. Plant Microbe Interact. (1994) [Pubmed]
  4. Co-evolution of the agrocinopine opines and the agrocinopine-mediated control of TraR, the quorum-sensing activator of the Ti plasmid conjugation system. Oger, P., Farrand, S.K. Mol. Microbiol. (2001) [Pubmed]
  5. Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer. Oger, P., Kim, K.S., Sackett, R.L., Piper, K.R., Farrand, S.K. Mol. Microbiol. (1998) [Pubmed]
  6. Identification of two quorum-sensing systems in Sinorhizobium meliloti. Marketon, M.M., González, J.E. J. Bacteriol. (2002) [Pubmed]
  7. A simple, rapid, sensitive method detecting homoserine lactone (HSL)-related compounds in microbial extracts. Singh, M.P., Greenstein, M. J. Microbiol. Methods (2006) [Pubmed]
  8. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. Fuqua, W.C., Winans, S.C. J. Bacteriol. (1994) [Pubmed]
  9. The Agrobacterium tumefaciens rnd homolog is required for TraR-mediated quorum-dependent activation of Ti plasmid tra gene expression. Luo, Z.Q., Farrand, S.K. J. Bacteriol. (2001) [Pubmed]
  10. Hierarchical gene regulatory systems arising from fortuitous gene associations: controlling quorum sensing by the opine regulon in Agrobacterium. Piper, K.R., Beck Von Bodman, S., Hwang, I., Farrand, S.K. Mol. Microbiol. (1999) [Pubmed]
 
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