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Gene Review

rom  -  RNAI modulator protein

Escherichia coli

 
 
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Disease relevance of rom

  • (ii) Specific overexpression of the rom gene abolished plasmid transformation into E. coli rho-15 [1].
  • Isolation and characterization of a ColE1-like plasmid from Enterobacter agglomerans with a novel variant of rom gene [2].
  • The pPIGDM1-derived rom-like coding sequence is also homologous to the rom-like gene present in the Proteus vulgaris plasmid pPvul [2].
 

High impact information on rom

  • Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein [3].
  • We propose that DnaC-A84T is proficient in the assembly of replication forks for both initiation of chromosome replication (at oriC) and replication restart via the Rep-PriC pathway, but that it is specifically defective for replication restart via the PriA-PriB pathway (and consequently also for replication of the Rom(-) ColE1-like plasmids) [4].
  • A model for the regulation of pBR322 plasmid DNA replication by Rom protein in amino acid-starved Escherichia coli strains is proposed [5].
  • Upon reduction of the protein with dithiothreitol, it disintegrated into assumingly identical smaller subunits (E. coli rom Mr 90-85,000 to 45-40,000 and S. cerevisiae from 52-49,500 to 28-29,500) [6].
  • In the cloned ColE1 type origin of replication of pTC a deletion was detected compared to other ColE1 replicons affecting the replication modulator gene rom [7].
 

Chemical compound and disease context of rom

  • Inactivation of the putative rom gene by insertion of a kanamycin-resistance cassette resulted in 4.5-fold increase in pEC156-derived plasmid copy number in E. coli cells [8].
 

Biological context of rom

  • Plasmid pBR322, as well as its delta rom and delta bla derivatives, were lost from their host within 60 generations, but a number of delta tet derivatives were quite stable under the same conditions [9].
  • Inactivation of the putative rom gene by insertion of an amplicillin-resistance gene resulted in significant increase in pPIGDM1-derived plasmid copy number in E. coli-despite the fact that amino acid sequence of the putative RNA 1 modulator (Rom) protein is only 55.7% identical to the ColE1 analog [2].
 

Other interactions of rom

  • All of these elements (RNA I, RNA II, and rom) reveal a high level of similarity to ColE1 homologs [8].

References

  1. Incompatibility of Escherichia coli rho mutants with plasmids is mediated by plasmid-specific transcription. Li, T.K., Panchenko, Y.A., Drolet, M., Liu, L.F. J. Bacteriol. (1997) [Pubmed]
  2. Isolation and characterization of a ColE1-like plasmid from Enterobacter agglomerans with a novel variant of rom gene. Mikiewicz, D., Wróbel, B., Wegrzyn, G., Płucienniczak, A. Plasmid (1997) [Pubmed]
  3. Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein. Tomizawa, J., Som, T. Cell (1984) [Pubmed]
  4. A dnaC mutation in Escherichia coli that affects copy number of ColE1-like plasmids and the PriA-PriB (but not Rep-PriC) pathway of chromosomal replication restart. Harinarayanan, R., Gowrishankar, J. Genetics (2004) [Pubmed]
  5. Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. Herman, A., Wegrzyn, A., Wegrzyn, G. Mol. Gen. Genet. (1994) [Pubmed]
  6. Properties of the purified APS-kinase from Escherichia coli and Saccharomyces cerevisiae. Schriek, U., Schwenn, J.D. Arch. Microbiol. (1986) [Pubmed]
  7. Characterization of an F18+ enterotoxigenic Escherichia coli strain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC. Olasz, F., Fekete, P.Z., Blum-Oehler, G., Boldogkoi, Z., Nagy, B. FEMS Microbiol. Lett. (2005) [Pubmed]
  8. Characterization of pEC156, a ColE1-type plasmid from Escherichia coli E1585-68 that carries genes of the EcoVIII restriction-modification system. Mruk, I., Sektas, M., Kaczorowski, T. Plasmid (2001) [Pubmed]
  9. Stability of pBR322-derived plasmids. Chiang, C.S., Bremer, H. Plasmid (1988) [Pubmed]
 
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