High impact information on CSN2

• We report that fission yeast Ddb1, Cullin 4 (Pcu4), and CSN subunits Csn1 and Csn2 are required for degradation of the ribonucleotide reductase (RNR) inhibitor protein Spd1 [1].
• The human CSN2 and STATH genes were not expressed in the transchromosomal mice [2].
• Using polymerase chain reaction on genomic DNA from somatic cell hybrids, we have localized the human beta-casein gene (CSN2) to 4pter----q21 [3].
• The nucleotide (nt) sequence contained an open reading frame sufficient to encode the entire amino-acid (aa) sequence of a beta-casein precursor protein consisting of 210 aa and a signal peptide of 15 aa [4].
• Epo treated ARO-81 cells only had an increase in CSN2 expression [5].

Biological context of CSN2

• The human beta-casein gene (CSN2) has been mapped by fluorescence in situ hybridization to human chromosome 4q13 --> q21 and to the homologous chimpanzee chromosome at 3p13 --> p12 [6].
• RFLP and linkage analysis of the porcine casein loci--CASAS1, CASAS2, CASB and CASK [7].
• The CASAS1, CASAS2 and CASB casein loci were also shown to be linked with no recombinant haplotypes observed amongst 77 meioses in Large White and Meishan F1 and F2 crosses [7].

Other interactions of CSN2

• On the one hand, the physico-chemical measures of interactions of signal peptides of casein beta and alpha s2 confirm their mutual genetic relationship, and on the other hand they show the divergence of casein beta and alpha s2 from casein kappa signal peptide [8].

Analytical, diagnostic and therapeutic context of CSN2

• By isotopic in situ hybridization, CSN2 was regionally localized to sheep Chr (OOV) 6q22-q31, anchoring this syntenic group of markers on to OOV6 and confirming its homology at a molecular and cytological level with cattle Chr 6 [9].

References