High impact information on rbp2a

• The cellular effects of RA are dependent upon the complement of nuclear receptors expressed (RARs and RXRs), which transduce retinoid signals into transcriptional regulation, the presence of cellular retinoid-binding proteins (CRABP and CRBP), which may be involved in RA metabolism, and the activity of RA metabolizing enzymes [1].
• Comparative analysis suggests that the duplicate genes of rbp1 and rbp2 in the zebrafish genome may have arisen by chromosomal or whole-genome duplication [2].
• RT-PCR detected mRNA transcripts in the adult intestine, liver, brain, ovary and testis for rbp1 gene and in the intestine and liver for rbp2 gene [3].
• Spatio-temporal distribution of cellular retinol-binding protein gene transcripts (CRBPI and CRBPII) in the developing and adult zebrafish (Danio rerio) [3].
• The zebrafish CRBPI gene (rbp1) and CRBPII gene (rbp2) both consist of four exons separated by three introns, identical to all other iLBP genes in vertebrates [3].

Biological context of rbp2a

• Two transcription start sites were identified in the rbp1 promoter and a single transcription start site was identified for rbp2 [3].
• Whole mount in situ hybridization also showed that the zebrafish rbp2 mRNA was localized specifically in the embryonic intestinal bulb and the developing intestine during the larval stage, implying a novel function for the rbp2 gene product during organogenesis and development of the zebrafish intestine [3].

Analytical, diagnostic and therapeutic context of rbp2a

• Radiation hybrid mapping revealed that the zebrafish and human CRBPII genes are located in syntenic groups [4].
• In situ hybridization and emulsion autoradiography localized the CRBPII mRNA to the intestine and the liver of adult zebrafish [4].

References