High impact information on Man1a2

• Two naturally occurring mouse alpha-1,2-mannosidase IB cDNA clones differ in three point mutations. Mutation of Phe592 to Ser592 is sufficient to abolish enzyme activity [1].
• The amino acid sequences of PCR1 and PCR2 were 88 and 65% identical with the corresponding sequence of the rabbit enzyme, respectively [2].
• Using PCR2 as a probe, a novel mouse cDNA was isolated from a 3T3 cDNA library [2].
• Fluorescence in situ hybridization with alpha 1,2-mannosidase IB genomic DNA (Cos.31.1) localized the gene to mouse chromosome 3F2 [3].
• Analysis of Cos.31.1 and the P1 clones showed that the alpha 1,2-mannosidase IB gene spans > or = 80 kb of the genome and consists of 13 exons representing the complete open reading frame of the enzyme [3].

Biological context of Man1a2

• PCR1 or PCR2 followed by sequencing or RFLP analysis not only detected Sarcocystis sporocysts in feces but also enabled to ascertain the genotype of the species as S. neurona [4].

Analytical, diagnostic and therapeutic context of Man1a2

• Southern blot analysis of mouse genomic DNA using PCR1 and PCR2 as probes revealed that they are derived from two different genes, indicating the existence of a mammalian mannosidase gene family with at least two members [2].

References