High impact information on Robo3

• The slit receptor Rig-1/Robo3 controls midline crossing by hindbrain precerebellar neurons and axons [1].
• Elevated levels of Rig-1 mRNA and protein were found in Rb-/- embryos [2].
• Rig-1 mRNA was transiently expressed in the embryonic hindbrain and spinal cord [2].
• The longest open reading frame of Rig-1 encoded a polypeptide that consists of a putative extracellular segment with five immunoglobulin-like domains and three fibronectin III-like domains, a putative transmembrane domain, and a distinct intracellular segment [2].
• Ectopic expression of a transmembrane form of Rig-1, but not the secreted form, promoted neuronal cell entrance to S phase and repressed the expression of a marker of differentiated neuron, Talpha1 tubulin [2].

Biological context of Robo3

• In summary, the ubiquitous tumor suppressor RB and the neuron-enriched transcription factor Pax-2 may play a role in the regulation of Rig-1 expression during embryogenesis [3].

Anatomical context of Robo3

• In the spinal cord Rig-1 shows overlapping but distinct pattern of expression with Robo1 and Robo2 [4].
• During their tangential migration, neurons of all major hindbrain precerebellar nuclei express high levels of Rig-1 mRNA [1].

Regulatory relationships of Robo3

• Rig-1 promoter activity is downregulated by RB and upregulated by Pax-2 [3].

Other interactions of Robo3

• Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development [4].
• Pax-2 interacts with RB and reverses its transcriptional suppression on the Rig-1 promoter [3].

References