Disease relevance of Ddef2

• Pyelonephritis-associated P-pili (PAP) of Escherichia coli O6,H(-),K1(-),F12,haemolysin(-) were purified by salt precipitation and affinity chromatography using Synsorb P1 [1].
• Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes [2].
• Serum amylase, pancreatic water content (as a measure of edema), pancreatic myeloperoxidase activity, and Reg/PAP expression were quantified [2].
• BACKGROUND: The cystic fibrosis (CF) mouse pancreas has constitutively elevated expression of the Reg/PAP cell stress genes (60-fold greater Reg3alpha, and 10-fold greater PAP/Reg3beta and Reg3gamma) [2].

High impact information on Ddef2

• We found that when loxP sites flank in a direct orientation the transcription termination sequence (1.1 kb) of the T. brucei GPEET/PAG3 locus, CRE recombinase deletes this termination sequence, permitting transcription and subsequent expression of a downstream reporter gene for the green fluorescent protein (GFP) [3].
• A monoclonal antibody (mAb) was produced by fusion of the PAI myeloma cell line with splenic lymphocytes from BALB/c mice immunised with the purified PAP [1].

Analytical, diagnostic and therapeutic context of Ddef2

• The mAb reacted with PAP in both immunofluorescence and haemagglutination tests when 108 strains isolated from urine samples were tested; the two tests were in agreement for 202 of 204 strains isolated from faecal samples [1].

References