High impact information on RALY

• In conclusion, we have found that P542 is a relatively weak promoter compared with P97 and may be downregulated in differentiated epithelial cells [1].
• We have also shown that the P542 promoter is downregulated by the transcription factor activator protein 4 (AP-4) and the differentiation-dependent factor hSkn-1a, both binding downstream of the transcription initiation site [1].
• The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC [2].
• The 542-bp nucleotide sequence of this element, named P542, was determined and a PCR assay was standardized for its amplification [3].
• The presence of the P542 element was confirmed in 11 T. rangeli isolates from mammalian hosts and insect vectors originating from several countries in Latin America. Negative amplification was observed with different T. cruzi strains and other trypanosomatids [3].

Anatomical context of RALY

• The potential field application of the P542 PCR assay was investigated in simulated samples containing T. rangeli and/or T. cruzi and intestinal tract and feces of Rhodnius prolixus [3].

Analytical, diagnostic and therapeutic context of RALY

• We confirmed by anchored RT-PCR our presumption that the 5' sequences of p542 also have a high degree of identity with Raly, including presence of RNA binding motifs characteristic of hnRNPs [4].

References