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Gene Review

POU2F3  -  POU class 2 homeobox 3

Homo sapiens

Synonyms: Epoc-1, OCT-11, OCT11, OTF-11, OTF11, ...
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Disease relevance of POU2F3


High impact information on POU2F3

  • In PA extracted from cells pretreated with 200 microM propranolol before stimulation with 1 microM FMLP, phospholipase A1 (PLA1)-digestion for 90 min, followed by quantitation of residual PA, showed that a minimum of 44% of PA in control (undigested) sample was diacyl-PA; alkylacyl-PA remained undigested by PLA1 [6].
  • The expression pattern and mutant phenotype suggest that the PLA1 gene acting in developing leaf primordia affects the timing of successive leaf initiation and the termination of vegetative growth [7].
  • The PLA1 epitope on platelet GPIIIa has a sulfhydryl-dependent conformation and is dependent on a leucine 33/proline33 polymorphism [8].
  • GPIIIa-related PLA1 antigens with different molecular weights: studies in platelets, endothelial cells, and megakaryocytes [9].
  • We conclude that the PLA1 antigen is present on a 120,000- as well as 100,000-mol wt glycoprotein of platelets and megakaryocytes, a 105,000-mol wt band of megakaryocytes, and a 100,000-mol wt glycoprotein of endothelial cells [9].

Biological context of POU2F3


Anatomical context of POU2F3

  • Immunohistochemical analysis on a cervical tissue microarray detected POU2F3 protein in the epithelium above the basal layer [10].
  • The Skn-1a POU transcription factor is primarily expressed in keratinocytes of murine embryonic and adult epidermis [11].
  • The Oct-11 gene is expressed during mouse embryogenesis and in the adult thymus and testis [12].
  • In HeLa cells transfected with circular HPV16 DNA along with the expression plasmid for hSkn-1a, the transcript from P(670) was detectable, which indicates that the results obtained with the reporter plasmids are likely to have mimicked the regulation of P(670) in authentic HPV16 DNA [13].
  • A similar differentiation-dependent distribution of expression has been reported for the recently cloned epithelial cell specific transcription factor Epoc-1/skn-1a [14].

Associations of POU2F3 with chemical compounds

  • Bisulfite sequencing analysis revealed that methylation of specific CpG sites (-287 to -70 bp) correlated with POU2F3 expression, which could be reactivated with a demethylating agent [10].
  • While maintained for several days in the presence of doxycycline, the HeLa/hSkn-1a cells showed a slightly prolonged time of population doubling, the occasional appearance of flat cells with lowered DNA synthesis, and a low level of apoptotic DNA fragmentation [15].
  • A colony inhibition assay showed that the growth of HeLa S3, SiHa, CaSki, and C-33A cells was repressed, as seen from the decreased number and average size of the drug-resistant colonies at 2 or 3 weeks after transfection with a plasmid that can express hSkn-1a and neomycin resistance gene [15].
  • Both mPA-PLA1beta and mPA-PLA1alpha recombinant proteins exhibited PA-specific PLA1 activity and were vanadate-sensitive [16].
  • Additionally, these data suggest that the combined actions of PLA1 and the 95-kDa protein generate significant amounts of free arachidonic acid in the brain [17].

Physical interactions of POU2F3

  • Skn-1a and Skn-1d1 bound the SPRR2A octamer site with comparable affinity and functioned as transcriptional activators [18].
  • YY1 complexing with a short DNA fragment having site 1 was displaced by hSkn-1a, indicating that hSkn-1a's affinity with site 1 was stronger than YY1's. Disrupting the binding sites by nucleotide substitutions raised the basal expression level of luciferase and decreased the enhancing effect of hSkn-1a [13].

Other interactions of POU2F3

  • Decreased expression of POU2F3 and TRIM29 was found in some cervical tumors and CC cell lines [19].
  • The POU domain proteins Oct1, Skn1a/i, and Oct6, which are abundantly expressed in the epidermal cells, act to both stimulate and repress transcription in a general and a cell type-specific mode [20].
  • Displacement of YY1 by differentiation-specific transcription factor hSkn-1a activates the P(670) promoter of human papillomavirus type 16 [13].

Analytical, diagnostic and therapeutic context of POU2F3

  • Monoclonal antibody LK-4 differentiates PLA1/PLA1 from PLA2/PLA2 platelet lysates on solid phase enzyme-linked immunosorbent assay (ELISA), as well as immunoblot [8].
  • Footprint analyses and gel retardation assays demonstrated direct binding of Epoc-1/skn-1a to a hitherto uncharacterized site within this region [14].
  • To clarify the role of PLA1/PLA2 polymorphism in coronary risk, a meta-analysis of published data was conducted [2].
  • Nevertheless, very low PLA1 activities (< or = 5 U/L, most likely due to heparin perfusion therapy) could also be detected by palmitic and stearic acid release from the sn-1 position, leading to small changes in fatty acid release patterns of sera with low PLA activities [21].
  • We find that the PLA1 antigen of stored blood is due to the presence of platelet fragments which can be removed by centrifugation and that divalent cation-chelating agents play no role in the apparent binding of these fragments to platelets [5].


  1. The Transcription Factor PLA-1/SKN-1A is Expressed in Human Placenta and Regulates the Placental Lactogen-3 Gene Expression. Jiménez-Mateo, O., Rodríguez-Torres, A., Avila, S., Castrillo, J.L. Placenta (2006) [Pubmed]
  2. Platelet glycoprotein receptor IIIa polymorphism PLA1/PLA2 and coronary risk: a meta-analysis. Di Castelnuovo, A., de Gaetano, G., Donati, M.B., Iacoviello, L. Thromb. Haemost. (2001) [Pubmed]
  3. Examination of POU homeobox gene expression in human breast cancer cells. Jin, T., Branch, D.R., Zhang, X., Qi, S., Youngson, B., Goss, P.E. Int. J. Cancer (1999) [Pubmed]
  4. Anti-HPA-3A induces severe neonatal alloimmune thrombocytopenia. Glade-Bender, J., McFarland, J.G., Kaplan, C., Porcelijn, L., Bussel, J.B. J. Pediatr. (2001) [Pubmed]
  5. Posttransfusion purpura: conversion of PLA1-negative platelets to the PLA1-positive phenotype by stored plasma is not due to the presence of soluble PLA1 antigen. Ehmann, W.C., Dancis, A., Ferziger, R., Karpatkin, S. Proc. Soc. Exp. Biol. Med. (1990) [Pubmed]
  6. Phosphatidic acid as a second messenger in human polymorphonuclear leukocytes. Effects on activation of NADPH oxidase. Agwu, D.E., McPhail, L.C., Sozzani, S., Bass, D.A., McCall, C.E. J. Clin. Invest. (1991) [Pubmed]
  7. PLASTOCHRON1, a timekeeper of leaf initiation in rice, encodes cytochrome P450. Miyoshi, K., Ahn, B.O., Kawakatsu, T., Ito, Y., Itoh, J., Nagato, Y., Kurata, N. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  8. Inhibition of binding of anti-PLA1 antibodies to platelets with monoclonal antibody LK-4. Evidence for multiple PLA1 receptor sites on platelet GPIIIa. Liu, L.X., Nardi, M.A., Casella, J.F., Karpatkin, S. Blood (1996) [Pubmed]
  9. GPIIIa-related PLA1 antigens with different molecular weights: studies in platelets, endothelial cells, and megakaryocytes. Dancis, A., Ehmann, C., Ferziger, R., Grima, K., Karpatkin, S. Blood (1988) [Pubmed]
  10. Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer. Zhang, Z., Huettner, P.C., Nguyen, L., Bidder, M., Funk, M.C., Li, J., Rader, J.S. Oncogene (2006) [Pubmed]
  11. Characterization of the regulatory domains of the human skn-1a/Epoc-1/Oct-11 POU transcription factor. Hildesheim, J., Foster, R.A., Chamberlin, M.E., Vogel, J.C. J. Biol. Chem. (1999) [Pubmed]
  12. Cloning, chromosomal localization and expression pattern of the POU domain gene Oct-11. Goldsborough, A.S., Healy, L.E., Copeland, N.G., Gilbert, D.J., Jenkins, N.A., Willison, K.R., Ashworth, A. Nucleic Acids Res. (1993) [Pubmed]
  13. Displacement of YY1 by differentiation-specific transcription factor hSkn-1a activates the P(670) promoter of human papillomavirus type 16. Kukimoto, I., Kanda, T. J. Virol. (2001) [Pubmed]
  14. Regulation of human papillomavirus transcription by the differentiation-dependent epithelial factor Epoc-1/skn-1a. Yukawa, K., Butz, K., Yasui, T., Kikutani, H., Hoppe-Seyler, F. J. Virol. (1996) [Pubmed]
  15. Keratinocyte-specific POU transcription factor hSkn-1a represses the growth of cervical cancer cell lines. Enomoto, Y., Enomoto, K., Kitamura, T., Kanda, T. Oncogene (2004) [Pubmed]
  16. Biochemical and molecular characterization of two phosphatidic acid-selective phospholipase A1s, mPA-PLA1alpha and mPA-PLA1beta. Hiramatsu, T., Sonoda, H., Takanezawa, Y., Morikawa, R., Ishida, M., Kasahara, K., Sanai, Y., Taguchi, R., Aoki, J., Arai, H. J. Biol. Chem. (2003) [Pubmed]
  17. Purification of a lysophospholipase from bovine brain that selectively deacylates arachidonoyl-substituted lysophosphatidylcholine. Pete, M.J., Exton, J.H. J. Biol. Chem. (1996) [Pubmed]
  18. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. Cabral, A., Fischer, D.F., Vermeij, W.P., Backendorf, C. J. Biol. Chem. (2003) [Pubmed]
  19. Fine mapping and evaluation of candidate genes for cervical cancer on 11q23. Zhang, Z., Gerhard, D.S., Nguyen, L., Li, J., Traugott, A., Huettner, P.C., Rader, J.S. Genes Chromosomes Cancer (2005) [Pubmed]
  20. Complex interactions between epidermal POU domain and activator protein 1 transcription factors regulate the expression of the profilaggrin gene in normal human epidermal keratinocytes. Jang, S.I., Karaman-Jurukovska, N., Morasso, M.I., Steinert, P.M., Markova, N.G. J. Biol. Chem. (2000) [Pubmed]
  21. Increased phospholipase A activities in sera of intensive-care patients show sn-2 specificity but no acyl-chain selectivity. Püttmann, M., Aufenanger, J., von Ochsenstein, E., Dürholt, S., van Ackern, K., Harenberg, J., Hoffmann, G.E. Clin. Chem. (1993) [Pubmed]
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