Disease relevance of map1

• Genetic and sequencing analysis indicated that the cloned gene is map1], which was originally defined by a mutation that caused h+-speciftic sterility [1].

High impact information on map1

• The fact that the map1/mam2 homozygous diploid cells are incapable of sporulation implies that the pheromone signalling system is necessary for sporulation in diploid cells [2].
• We cloned the Schizosaccharomyces pombe map1 gene by virtue of its ability to stimulate transcription of the sxa2 gene, which encodes a carboxypeptidase expressed specifically in h- cells in response to mating-pheromone signaling [1].
• Schizosaccharomyces pombe map1+ encodes a MADS-box-family protein required for cell-type-specific gene expression [1].
• Overexpression of map1 resulted in enhanced transcription of cell-type-specilic genes, but the range of genes affected by Map1 was restricted by the mating type of the cell [1].
• The mei2 gene encoding a positive regulator for meiosis was normally transcribed in the map1 mutant, suggesting that the map1 function was specific to mat1-Pi [3].

Biological context of map1

• Northern (RNA) blot analysis showed that the function of map1 is absolutely essential for the expression of h+-specific genes and is required for the full activation of h--specific gene expression [1].

References