Disease relevance of RAI14

• Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium [1].

High impact information on RAI14

• NORPEG (RAI14), a developmentally regulated gene induced by retinoic acid, encodes a 980 amino acid (aa) residue protein containing six ankyrin repeats and a long coiled-coil domain [Kutty et al., J. Biol. Chem. 276 (2001), pp. 2831-2840] [2].
• Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain [1].
• Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells [1].
• Confocal immunofluorescence analysis showed that the subcellular localization of NORPEG in retinal pigment epithelial (ARPE-19) cells varies with cell density, with predominantly nuclear localization in nonconfluent cells, but a cytoplasmic localization, reminiscent of cytoskeleton, in confluent cultures [2].
• Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes [3].

Analytical, diagnostic and therapeutic context of RAI14

• Primary cultures of Müller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy [1].
• One of these clones was a short transcript of NORPEG which we proceeded to analyze by RT-PCR [3].

References