Disease relevance of SNORD32A

• Glutamate synthase was purified about 250-fold from Nocardia mediterranei U32 and characterized [1].
• A mini-chromosome constructed by cloning the putative U32 oriC DNA fragment into an Escherichia coli plasmid was able to replicate autonomously, but was unstable, in A. mediterranei U32 with an estimated copy number of two per cell [2].

High impact information on SNORD32A

• We have identified nine additional members of this family, U32 to U40 [3].
• Our results suggest that the purine-rich J(23) strand is antiparallel to the D5 strand containing U32 and U33 [4].
• Cloning and characterization of the chromosomal replication origin region of Amycolatopsis mediterranei U32 [2].
• In contrast, U32/CD3/28 induced high levels of CD4 T-cell thymidine uptake but were unable to sustain long-term T-cell expansion [5].
• The primary results suggested that amrB-amkB genes might be involved in the regulation of central carbohydrate metabolism in A. mediterranei U32 [6].

Biological context of SNORD32A

• To further investigate the possible physiological function of the moeA gene, a double crossover gene replacement was achieved by inserting an aparmycin resistance gene into moeA in the A. mediterranei U32 chromosome [7].
• A novel two-component signal transduction system amrB-amkB was cloned from rifamycin SV-producing Amycolatopsis mediterranei U32, and their biochemical functions as a response regulator and a histidine protein kinase, respectively, were proven [6].

References