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Gene Review:

UL26.5 - clipped near C terminus

Human herpesvirus 1

Oien, N.L. et al., Desai, P. et al., Person, S. et al., Eberle, R. et al., Kennard, J. et al., et al.

Haanes, Chen, Lowery,

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Disease relevance of UL26.5

VP22a was shown to form the inner core of the B capsid, since in infections in which the UL26.5 gene was omitted the 100-nm capsids that formed lacked the inner core [1].
An essential step in assembly of herpes simplex virus (HSV) type 1 capsids involves interaction of the major capsid protein (VP5) with the C terminus of the scaffolding protein (encoded by the UL26.5 gene) [2].
In these studies, we tested whether the HCMV UL80.5 gene would substitute for the HSV UL26.5 gene in a baculovirus capsid assembly system that we have previously described (D. R. Thomsen et al., J. Virol. 68:2442-2457, 1994) [2].
Previous studies have shown that the bovine herpesvirus 1 (alphaherpesvirus) UL26.5 homolog will functionally substitute for the HSV UL26.5 gene (E. J. Haanes et al., J. Virol. 69:7375-7379, 1995) [2].
The homolog of the UL26.5 gene in the human cytomegalovirus (HCMV) genome is the UL80.5 gene [2].

High impact information on UL26.5

Formation of portal-containing capsids minimally requires the four structural proteins (VP5, VP19C, VP23, and UL6) and a scaffolding protein (UL26.5) [3].
In contrast to UL26.5, increased expression of UL26 did not compensate for the DeltaICP35 growth defect [4].
VP5, the major capsid protein of herpes simplex virus type 1 (HSV-1), interacts with the C-terminal residues of the scaffold molecules encoded by the overlapping UL26 and UL26.5 open reading frames [5].
Herpes simplex virus type 1 (HSV-1) polypeptides specified by overlapping genes UL26 and UL26.5 form a scaffold around which the icosahedral capsid shell is assembled [6].
The recombinant was shown to express the SA8 gB and p40 proteins (UL27 & UL26.5 gene products, respectively); all other proteins were indistinguishable from those of HSV1 [7].

Biological context of UL26.5

A previously isolated virus (KUL26-610/611) was used that encoded a lethal mutation in the UL26 and UL26.5 open reading frames and required a transformed cell line that expresses these proteins for virus growth [5].
Nucleotide sequence and transcriptional analysis of a portion of the bovine herpesvirus genome encoding genes homologous to HSV-1, UL25, UL26 and UL26.5 [8].

Other interactions of UL26.5

Capsids are formed in a mutant virus blocked at the maturation site of the UL26 and UL26.5 open reading frames of herpes simplex virus type 1 but are not formed in a null mutant of UL38 (VP19C) [9].

References

Assembly of herpes simplex virus (HSV) intermediate capsids in insect cells infected with recombinant baculoviruses expressing HSV capsid proteins. Thomsen, D.R., Roof, L.L., Homa, F.L. J. Virol. (1994) [Pubmed]
Assembly of herpes simplex virus capsids using the human cytomegalovirus scaffold protein: critical role of the C terminus. Oien, N.L., Thomsen, D.R., Wathen, M.W., Newcomb, W.W., Brown, J.C., Homa, F.L. J. Virol. (1997) [Pubmed]
Identification of a region in the herpes simplex virus scaffolding protein required for interaction with the portal. Singer, G.P., Newcomb, W.W., Thomsen, D.R., Homa, F.L., Brown, J.C. J. Virol. (2005) [Pubmed]
Evidence for controlled incorporation of herpes simplex virus type 1 UL26 protease into capsids. Sheaffer, A.K., Newcomb, W.W., Brown, J.C., Gao, M., Weller, S.K., Tenney, D.J. J. Virol. (2000) [Pubmed]
Second site mutations in the N-terminus of the major capsid protein (VP5) overcome a block at the maturation cleavage site of the capsid scaffold proteins of herpes simplex virus type 1. Desai, P., Person, S. Virology (1999) [Pubmed]
The 25 amino acid residues at the carboxy terminus of the herpes simplex virus type 1 UL26.5 protein are required for the formation of the capsid shell around the scaffold. Kennard, J., Rixon, F.J., McDougall, I.M., Tatman, J.D., Preston, V.G. J. Gen. Virol. (1995) [Pubmed]
Genetic and functional complementation of the HSV1 UL27 gene and gB glycoprotein by simian alpha-herpesvirus homologs. Eberle, R., Tanamachi, B., Black, D., Blewett, E.L., Ali, M., Openshaw, H., Cantin, E.M. Arch. Virol. (1997) [Pubmed]
Nucleotide sequence and transcriptional analysis of a portion of the bovine herpesvirus genome encoding genes homologous to HSV-1, UL25, UL26 and UL26.5. Haanes, E.J., Chen, C.C., Lowery, D.E. Virus Res. (1997) [Pubmed]
Capsids are formed in a mutant virus blocked at the maturation site of the UL26 and UL26.5 open reading frames of herpes simplex virus type 1 but are not formed in a null mutant of UL38 (VP19C). Person, S., Desai, P. Virology (1998) [Pubmed]

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