Disease relevance of UL3.5

• Identification and characterization of the pseudorabies virus UL3.5 protein, which is involved in virus egress [1].
• Functional complementation of UL3.5-negative pseudorabies virus by the bovine herpesvirus 1 UL3.5 homolog [2].
• The UL3.5 gene is positionally conserved but highly variable in size and sequence in different members of the Alphaherpesvirinae and is absent from herpes simplex virus genomes [2].
• The fourth open reading frame, UL3.5, is not present in the HSV-1 genome [3].
• We have shown previously that the pseudorabies virus (PrV) UL3.5 gene encodes a nonstructural protein which is required for secondary envelopment of intracytoplasmic virus particles in the trans-Golgi region [2].

High impact information on UL3.5

• To assay functional complementation by a heterologous herpesviral UL3.5 protein, the UL3.5 gene of bovine herpesvirus 1 (BHV-1) was inserted at two different sites within the genome of UL3.5-negative PrV [2].

Analytical, diagnostic and therapeutic context of UL3.5

• Expression of BHV-1 UL3.5 compensated for the lack of PrV UL3.5, resulting in a ca. 1,000-fold increase in virus titer and restoration of plaque formation in cell culture [2].

References