Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

UL1 - contains a signal peptide; complexed with...

Bovine herpesvirus 1

Fuchs, W. et al., Khattar, S.K. et al., Chung, Y.T. et al., Fuchs, W. et al., Khattar, S.K. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of UL1

The UL1 ORF of 158 amino acids exhibits limited homology with UL1 (glycoprotein gL) of HSV-1 (27%) and PRV (21%) [1].
A derivative of bovine herpesvirus 1 (BoHV-1) UL3.5 lacking the last forty amino acids inhibits replication of BoHV-1 [2].
DNA sequence analysis of the bovine herpesvirus-1 (BHV-1) genome revealed the presence of an open reading frame named UL1 which exhibited limited homology to glycoprotein gL of herpes simplex virus-1 (S. K. Khattar, S. van Drunen Littel-van den Hurk, L. A. Babiuk, and S. K. Tikoo, Virology 213, 28-37) [3].
In the absence of UL3.5 protein, naked nucleocapsids accumulate in the cytoplasm, release of infectious virions is drastically reduced, and plaque formation in cell culture is inhibited (W. Fuchs, B. G. Klupp, H. Granzow, H.-J. Rziha, and T. C. Mettenleiter, J. Virol. 70:3517-3527, 1996) [4].
To identify and characterize the predicted 224 amino acid UL3.5 protein of PrV, a rabbit antiserum was prepared against a UL3.5 fusion protein expressed in Escherichia coli [5].

High impact information on UL1

Among the exceptions is the UL3.5 gene of pseudorabies virus (PrV) for which positional homologs have been detected in the genomes of varicella-zoster virus, equine herpesvirus 1, and bovine herpesvirus 1 but not in the genomes of herpes simplex virus types 1 and 2 [5].
This defect could be compensated by propagation on complementing UL3.5-expressing cell lines [5].
Our results thus demonstrate that the PrV UL3.5 gene encodes a nonstructural protein which plays an important role in virus replication, presumably during virus egress [5].
Enzymatic deglycosylation and lectin binding assays confirmed that the BHV-1 UL1 protein contains only O-linked oligosaccharides and was named glycoprotein gL [3].
However, only one polyadenylation signal was detected downstream of the UL3.5 ORF [1].

Biological context of UL1

The UL2 open reading frame of bovine herpesvirus 1 encodes a uracil-DNA glycosylase [6].

Analytical, diagnostic and therapeutic context of UL1

Sequence analysis within the unique long segment of the bovine herpesvirus 1 (BHV-1) genome previously identified an open reading frame (ORF), designated UL2, whose deduced polypeptide of 204 amino acids contained a consensus uracil-DNA glycosylase (UDGase) signature sequence [6].

References

Identification and transcriptional analysis of a 3'-coterminal gene cluster containing UL1, UL2, UL3, and UL3.5 open reading frames of bovine herpesvirus-1. Khattar, S.K., van Drunen Littel-van den Hurk, S., Babiuk, L.A., Tikoo, S.K. Virology (1995) [Pubmed]
A derivative of bovine herpesvirus 1 (BoHV-1) UL3.5 lacking the last forty amino acids inhibits replication of BoHV-1. Lam, N., Letchworth, G. Arch. Virol. (2004) [Pubmed]
Identification and characterization of a bovine herpesvirus-1 (BHV-1) glycoprotein gL which is required for proper antigenicity, processing, and transport of BHV-1 glycoprotein gH. Khattar, S.K., van Drunen Littel-van den Harke, S., Attah-Poku, S.K., Babiuk, L.A., Tikoo, S.K. Virology (1996) [Pubmed]
Functional complementation of UL3.5-negative pseudorabies virus by the bovine herpesvirus 1 UL3.5 homolog. Fuchs, W., Granzow, H., Mettenleiter, T.C. J. Virol. (1997) [Pubmed]
Identification and characterization of the pseudorabies virus UL3.5 protein, which is involved in virus egress. Fuchs, W., Klupp, B.G., Granzow, H., Rziha, H.J., Mettenleiter, T.C. J. Virol. (1996) [Pubmed]
The UL2 open reading frame of bovine herpesvirus 1 encodes a uracil-DNA glycosylase. Chung, Y.T., Hsu, W. Microbiol. Immunol. (1996) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.