The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

eng2a  -  engrailed homeobox 2a

Danio rerio

Synonyms: ENG-2, Homeobox protein en-2a, Homeobox protein engrailed-2a, SO:0000704, ZF-EN-2, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on eng2a

  • We also show that, consistent with results obtained using other methods, uncaging eng2a (which encodes the transcription factor Engrailed2a) in the head region during early development causes a severe reduction in the size of the eye and enhanced development of the midbrain and the midbrain-hindbrain boundary at the expense of the forebrain [1].
  • In situ hybridizations demonstrate that antibody injection causes downregulation of pax[b] transcripts in the posterior midbrain and alteration of wnt-1 and eng-2 expression in this area [2].
  • Thus, Eng2/Eng3 and Fgf8 are necessary to maintain midbrain identity in the neural plate and thereby position the DMB [3].
  • Transplantations, mRNA injections and morpholino knock-down experiments show that this feedback regulation of pax2.1 transcription occurs cell-autonomously, and that it requires eng2 and eng3 as known targets for Pax2.1 regulation [4].
  • The relative organization of ache, eng2, and shh genes is conserved between zebrafish and mammals and defines a synteny [5].

Biological context of eng2a

  • Consistent with this model, we demonstrate that zebrafish Engrailed and Pbx interact in vitro and that this interaction is required for both the eng2a overexpression phenotype and Engrailed's role in patterning the MHB [6].

Anatomical context of eng2a

  • A second site of activity was observed in each somite where specific myotomal cells, the muscle pioneers, express eng-2 [7].
  • In the hindbrain, eng-2 expression seems restricted to the primordium of the cerebellum [7].
  • The data are consistent with there being a temporal and spatial requirement for Hsp90 function within somitic cells which is necessary for the formation of eng-2-expressing muscle pioneers and possibly other striated muscle fiber types [8].

Associations of eng2a with chemical compounds


Other interactions of eng2a

  • We find that Fgf8 is a candidate for this signal, as it is both necessary and sufficient to repress pax6.1 and hence to shift the DMB anteriorly independently of the expression status of eng2/eng3 [3].

Analytical, diagnostic and therapeutic context of eng2a


  1. Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos. Ando, H., Furuta, T., Tsien, R.Y., Okamoto, H. Nat. Genet. (2001) [Pubmed]
  2. Zebrafish pax[b] is involved in the formation of the midbrain-hindbrain boundary. Krauss, S., Maden, M., Holder, N., Wilson, S.W. Nature (1992) [Pubmed]
  3. Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon. Scholpp, S., Lohs, C., Brand, M. Development (2003) [Pubmed]
  4. A novel positive transcriptional feedback loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines. Picker, A., Scholpp, S., Böhli, H., Takeda, H., Brand, M. Development (2002) [Pubmed]
  5. Zebrafish acetylcholinesterase is encoded by a single gene localized on linkage group 7. Gene structure and polymorphism; molecular forms and expression pattern during development. Bertrand, C., Chatonnet, A., Takke, C., Yan, Y.L., Postlethwait, J., Toutant, J.P., Cousin, X. J. Biol. Chem. (2001) [Pubmed]
  6. Pbx proteins cooperate with Engrailed to pattern the midbrain-hindbrain and diencephalic-mesencephalic boundaries. Erickson, T., Scholpp, S., Brand, M., Moens, C.B., Jan Waskiewicz, A. Dev. Biol. (2007) [Pubmed]
  7. Structure and early embryonic expression of the zebrafish engrailed-2 gene. Fjose, A., Njølstad, P.R., Nornes, S., Molven, A., Krauss, S. Mech. Dev. (1992) [Pubmed]
  8. Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90. Lele, Z., Hartson, S.D., Martin, C.C., Whitesell, L., Matts, R.L., Krone, P.H. Dev. Biol. (1999) [Pubmed]
WikiGenes - Universities