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Gene Review:

cadF - fibronectin-binding protein

Campylobacter jejuni RM1221

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Disease relevance of cadF

As a model, cadF gene of Campylobacter jejuni has been amplified on the microchip [1].
In spite of our success in purifying the N-terminal domain of the CadF protein, repeated attempts to express and purify the entire cadF gene in E. coli failed [2].

High impact information on cadF

The goal of this study was to develop specific detection assays for C. jejuni and C. coli isolates based on the cadF virulence gene and its product [3].
The cadF gene from C. jejuni and C. coli encodes a 37-kDa outer membrane protein that promotes the binding of these pathogens to intestinal epithelial cells [3].
Using a novel approach, we found it possible to express the entire cadF gene fused to a hexa-histidine encoding nucleotide sequence in C. jejuni [2].
One hundred seventeen (100%) isolates were positive for flaA, cadF, and ceuE gene primers [4].
The cadF, flaA, ceuE and cdtB genes were detected from 100% of the isolates [5].

Anatomical context of cadF

In contrast, the cadF mutant was not recovered from any of 60 chicks challenged, indicating that disruption of the cadF gene renders C. jejuni incapable of colonizing the cecum [6].

Analytical, diagnostic and therapeutic context of cadF

These findings indicate that the cadF gene and its product are conserved among C. jejuni and C. coli isolates and that a PCR assay based on the cadF gene may be useful for the detection of Campylobacter organisms in food products [3].
Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni [7].
2-DE followed by Far Western Blot analysis using fibronectin as a probe revealed a 29-kDa fibronectin-binding protein, designated here AdpB [8].

References

Towards a portable microchip system with integrated thermal control and polymer waveguides for real-time PCR. Wang, Z., Sekulovic, A., Kutter, J.P., Bang, D.D., Wolff, A. Electrophoresis (2006) [Pubmed]
Expression and purification of native and truncated forms of CadF, an outer membrane protein of Campylobacter. Mamelli, L., Pagès, J.M., Konkel, M.E., Bolla, J.M. Int. J. Biol. Macromol. (2006) [Pubmed]
Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. Konkel, M.E., Gray, S.A., Kim, B.J., Garvis, S.G., Yoon, J. J. Clin. Microbiol. (1999) [Pubmed]
Detection of seven virulence and toxin genes of Campylobacter jejuni isolates from Danish turkeys by PCR and cytolethal distending toxin production of the isolates. Bang, D.D., Borck, B., Nielsen, E.M., Scheutz, F., Pedersen, K., Madsen, M. J. Food Prot. (2004) [Pubmed]
PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates. Bang, D.D., Nielsen, E.M., Scheutz, F., Pedersen, K., Handberg, K., Madsen, M. J. Appl. Microbiol. (2003) [Pubmed]
The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Ziprin, R.L., Young, C.R., Stanker, L.H., Hume, M.E., Konkel, M.E. Avian Dis. (1999) [Pubmed]
Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Konkel, M.E., Garvis, S.G., Tipton, S.L., Anderson, D.E., Cieplak, W. Mol. Microbiol. (1997) [Pubmed]
Identification and characterization of a cell surface protein of Prevotella intermedia 17 with broad-spectrum binding activity for extracellular matrix proteins. Yu, F., Iyer, D., Anaya, C., Lewis, J.P. Proteomics (2006) [Pubmed]

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