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Gene Review:

myoB - class I myosin

Dictyostelium discoideum AX4

Temesvari, L.A. et al., Neuhaus, E.M. et al., Wessels, D. et al., Fukui, Y. et al., Peterson, M.D. et al.

Fukui, Inoué,

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High impact information on myoB

Cells lacking MyoB (myoA(-)/B(-), and myoB(-) cells) and wild-type cells treated with the myosin inhibitor butanedione monoxime accumulated a plasma membrane marker and biotinylated surface proteins on intracellular endocytic vacuoles [1].
Here, we demonstrate that Dictyostelium discoideum myosin IB functions in the recycling of plasma membrane components from endosomes back to the cell surface [1].
No alteration in contractile vacuole structure or function was observed in the myoB-, myoC- or myoB-/C- cell lines [2].
However, the myoB- and myoB-/C- cell lines, but not the myoC- cell line, were found to oversecrete the lysosomal enzymes alpha-mannosidase and acid phosphatase, during growth and starvation [2].
The rules and characteristics of nuclear behavior are demonstrated to be intact in two mutants affecting pseudopod formation, a myosin IB null mutant (myoB-) and a myosin II heavy chain phosphorylation mutant (3XALA) [3].

Biological context of myoB

Various properties of the endosomal pathway in the myoB-/C- double mutant as well as in the myoB- and myoC- single mutants, including intravesicular pH, and intracellular retention time and exocytosis of a fluid phase marker, were found to be indistinguishable from wild-type parental cells [2].

Anatomical context of myoB

These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton [4].

Analytical, diagnostic and therapeutic context of myoB

We show by indirect immunofluorescence that the knobby feet contain a high concentration of filamentous (F-) actin, myoB (a member of Dictyostelium myosin-I family), and alpha-actinin (an actin-binding protein) [5].

References

A myosin I is involved in membrane recycling from early endosomes. Neuhaus, E.M., Soldati, T. J. Cell Biol. (2000) [Pubmed]
Examination of the endosomal and lysosomal pathways in Dictyostelium discoideum myosin I mutants. Temesvari, L.A., Bush, J.M., Peterson, M.D., Novak, K.D., Titus, M.A., Cardelli, J.A. J. Cell. Sci. (1996) [Pubmed]
A computer-assisted system for reconstructing and interpreting the dynamic three-dimensional relationships of the outer surface, nucleus and pseudopods of crawling cells. Wessels, D., Voss, E., Von Bergen, N., Burns, R., Stites, J., Soll, D.R. Cell Motil. Cytoskeleton (1998) [Pubmed]
F-actin distribution of Dictyostelium myosin I double mutants. Peterson, M.D., Titus, M.A. J. Eukaryot. Microbiol. (1994) [Pubmed]
Amoeboid movement anchored by eupodia, new actin-rich knobby feet in Dictyostelium. Fukui, Y., Inoué, S. Cell Motil. Cytoskeleton (1997) [Pubmed]

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