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Gene Review

Drep-4  -  DNA fragmentation factor-related protein 4

Drosophila melanogaster

Synonyms: CAD, CG9414, DREP-4, Dmel\CG9414, Drep4, ...
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High impact information on Rep4

  • Biochemical fractionation and immunoprecipitation of dICAD from S2 cell extracts indicated that dICAD is complexed with dCAD in proliferating cells [1].
  • Using primers based on the amino acid sequence of the purified proteins, a cDNA coding for Drosophila CAD (dCAD) was cloned [2].
  • When a Drosophila neuronal cell line was induced to apoptosis by treatment with a kinase inhibitor, both dCAD and dICAD were cleaved [2].
  • In contrast, dCAD lacked the corresponding sequence, and the purified dCAD did not cause DNA fragmentation in nuclei in a cell-free system [2].
  • Enzymatic activities of aspartate transcarbamylase, carbamyl phosphate synthetase and dihydro-orotase, borne by the same multifunctional protein, CAD, are increased 6-12-fold in these resistant clones compared with parental cells [3].

Other interactions of Rep4

  • To identify the inhibitor, we tested recombinant DREP-1, which was previously identified using the Drosophila EST data base and found it also inhibited dCAD DNase [1].

Analytical, diagnostic and therapeutic context of Rep4

  • In addition, dCAD was also cleaved by these caspases, and behaved as a (p32)(2)(p20)(2) complex in gel filtration [2].
  • The data from immunotitration and immunoblotting experiments indicate that the increased enzyme activities result from the overproduction of CAD [3].


  1. Identification and developmental expression of inhibitor of caspase-activated DNase (ICAD) in Drosophila melanogaster. Mukae, N., Yokoyama, H., Yokokura, T., Sakoyama, Y., Sakahira, H., Nagata, S. J. Biol. Chem. (2000) [Pubmed]
  2. A novel activation mechanism of caspase-activated DNase from Drosophila melanogaster. Yokoyama, H., Mukae, N., Sakahira, H., Okawa, K., Iwamatsu, A., Nagata, S. J. Biol. Chem. (2000) [Pubmed]
  3. Overproduction of the first three enzymes of pyrimidine nucleotide biosynthesis in Drosophila cells resistant to N-phosphonacetyl-L-aspartate. Laval, M., Azou, Y., Giorgi, D., Rosset, R. Exp. Cell Res. (1986) [Pubmed]
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