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Gene Review

DFFB  -  DNA fragmentation factor, 40kDa, beta...

Homo sapiens

Synonyms: CAD, CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, ...
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Disease relevance of DFFB


Psychiatry related information on DFFB

  • Information from the lateral radiograph is routinely incorporated by radiologists in their decision-making process, and thus computer analysis of lateral images may potentially add another dimension to current CAD schemes [6].
  • PURPOSE: The primary objectives were to 1) examine the dose-response relationship between physical activity and mortality in individuals with CAD, and 2) determine whether the aforementioned relationship is consistent within strata of other personal characteristics [7].
  • In vivo imaging biomarkers of risk and successful risk reduction are therefore sought, but the information on possible in vivo imaging biomarkers is less mature than activities in CAD [8].
  • Incidence of Stress and Psychosocial Factors on Musculoskeletal Disorders in CAD and Data Entry [9].
  • PURPOSE: Despite many smoking cessation programs, many patients with CAD continue to smoke or re-smoke [10].

High impact information on DFFB

  • Furthermore, we demonstrate that the CIDE-N of CIDE-B regulates enzymatic activity of the DFF40/ DFF45 complex in vitro [11].
  • The extra DNA is usually linked to the short arm of chromosome B9, which retains CAD at its normal site [12].
  • We propose that, in the initial event of CAD gene amplification, much of the short arm is transferred from one B9 chromosome to another [12].
  • Using this assay, inverted duplications were detected in amplified DNA (both DM and HSR configurations) containing the myc gene (16-50 copies/cell) in four human tumor cell lines and in amplified DNA containing the CAD gene (30-200 copies/cell) in three PALA-resistant BHK cell lines [13].
  • The flies deficient in the ICAD gene did not express CAD, and did not undergo apoptotic DNA fragmentation during embryogenesis and oogenesis [14].

Chemical compound and disease context of DFFB


Biological context of DFFB


Anatomical context of DFFB


Associations of DFFB with chemical compounds

  • Using DNA-binding assay, we show that curcumin does not disrupt the DNA-DFF40/CAD interaction [22].
  • Activation and accumulation of 40-50 kD DFF40-like DNases in nuclei of palmitate-treated apoptotic MCF-7 were detected by SDS-DNA-PAGE assay [24].
  • CAD immobilization was mediated by its NH2 terminus independently of its DNA-binding activity and correlated with its association to the interchromosomal space [25].
  • Alternatively spliced CAD transcripts with fusion of exon 1 to exon 7 were isolated in most of the examined samples including HCC cells and normal controls [2].
  • Merbarone induces activation of caspase-activated DNase and excision of chromosomal DNA loops from the nuclear matrix [26].

Physical interactions of DFFB

  • These results suggest that Hsp70 binds free CAD in TCR-stimulated T cells to stabilize and augment its activity [19].
  • DNA topoisomerase IIalpha interacts with CAD nuclease and is involved in chromatin condensation during apoptotic execution [27].

Regulatory relationships of DFFB


Other interactions of DFFB


Analytical, diagnostic and therapeutic context of DFFB


  1. Structure and mutation analysis of the gene encoding DNA fragmentation factor 40 (caspase-activated nuclease), a candidate neuroblastoma tumour suppressor gene. Judson, H., van Roy, N., Strain, L., Vandesompele, J., Van Gele, M., Speleman, F., Bonthron, D.T. Hum. Genet. (2000) [Pubmed]
  2. Aberrant caspase-activated DNase (CAD) transcripts in human hepatoma cells. Hsieh, S.Y., Liaw, S.F., Lee, S.N., Hsieh, P.S., Lin, K.H., Chu, C.M., Liaw, Y.F. Br. J. Cancer (2003) [Pubmed]
  3. Renal carcinoma cells undergo apoptosis without oligonucleosomal DNA fragmentation. Yamaguchi, K., Uzzo, R., Dulin, N., Finke, J.H., Kolenko, V. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  4. Attenuated expression of DFFB is a hallmark of oligodendrogliomas with 1p-allelic loss. McDonald, J.M., Dunmire, V., Taylor, E., Sawaya, R., Bruner, J., Fuller, G.N., Aldape, K., Zhang, W. Mol. Cancer (2005) [Pubmed]
  5. Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis. Role of poly(ADP-ribose) polymerase-1 cleavage in endonuclease activation. Boulares, A.H., Zoltoski, A.J., Contreras, F.J., Yakovlev, A.G., Yoshihara, K., Smulson, M.E. J. Biol. Chem. (2002) [Pubmed]
  6. Automated lung segmentation in digital lateral chest radiographs. Armato, S.G., Giger, M.L., Ashizawa, K., MacMahon, H. Medical physics. (1998) [Pubmed]
  7. Influence of physical activity on mortality in elderly with coronary artery disease. Janssen, I., Jolliffe, C.J. Medicine and science in sports and exercise. (2006) [Pubmed]
  8. Improved small volume lung cancer detection with computer-aided detection: database characteristics and imaging of response to breast cancer risk reduction strategies. Freedman, M. Ann. N. Y. Acad. Sci. (2004) [Pubmed]
  9. Incidence of Stress and Psychosocial Factors on Musculoskeletal Disorders in CAD and Data Entry. Cail, F., Aptel, M. International journal of occupational safety and ergonomics : JOSE. (2005) [Pubmed]
  10. Decisional balances and the process of change in smoking cessation in patients with coronary artery diseases. Son, H.M. Taehan Kanho Hakhoe chi (2003) [Pubmed]
  11. Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis. Lugovskoy, A.A., Zhou, P., Chou, J.J., McCarty, J.S., Li, P., Wagner, G. Cell (1999) [Pubmed]
  12. Distinctive chromosomal structures are formed very early in the amplification of CAD genes in Syrian hamster cells. Smith, K.A., Gorman, P.A., Stark, M.B., Groves, R.P., Stark, G.R. Cell (1990) [Pubmed]
  13. Large inverted duplications are associated with gene amplification. Ford, M., Fried, M. Cell (1986) [Pubmed]
  14. Activation of the innate immunity in Drosophila by endogenous chromosomal DNA that escaped apoptotic degradation. Mukae, N., Yokoyama, H., Yokokura, T., Sakoyama, Y., Nagata, S. Genes Dev. (2002) [Pubmed]
  15. Comparative activity and toxicity of cis-diamminedichloroplatinum (DDP) and a combination of doxorubicin, cyclophosphamide, and DDP in disseminated transitional cell carcinomas of the urinary tract. Khandekar, J.D., Elson, P.J., DeWys, W.D., Slayton, R.E., Harris, D.T. J. Clin. Oncol. (1985) [Pubmed]
  16. Hypoxia-inducible factor (HIF) asparagine hydroxylase is identical to factor inhibiting HIF (FIH) and is related to the cupin structural family. Hewitson, K.S., McNeill, L.A., Riordan, M.V., Tian, Y.M., Bullock, A.N., Welford, R.W., Elkins, J.M., Oldham, N.J., Bhattacharya, S., Gleadle, J.M., Ratcliffe, P.J., Pugh, C.W., Schofield, C.J. J. Biol. Chem. (2002) [Pubmed]
  17. Molecular mechanism of inhibitory aryl hydrocarbon receptor-estrogen receptor/Sp1 cross talk in breast cancer cells. Khan, S., Barhoumi, R., Burghardt, R., Liu, S., Kim, K., Safe, S. Mol. Endocrinol. (2006) [Pubmed]
  18. Coronary microcirculatory vasoconstriction during ischemia in patients with unstable angina. Marzilli, M., Sambuceti, G., Fedele, S., L'Abbate, A. J. Am. Coll. Cardiol. (2000) [Pubmed]
  19. Heat shock protein 70 binds caspase-activated DNase and enhances its activity in TCR-stimulated T cells. Liu, Q.L., Kishi, H., Ohtsuka, K., Muraguchi, A. Blood (2003) [Pubmed]
  20. Activation of apoptotic and inflammatory pathways in dysfunctional donor hearts. Birks, E.J., Yacoub, M.H., Burton, P.S., Owen, V., Pomerance, A., O'Halloran, A., Banner, N.R., Khaghani, A., Latif, N. Transplantation (2000) [Pubmed]
  21. Apoptosis induction by T-2 toxin: activation of caspase-9, caspase-3, and DFF-40/CAD through cytosolic release of cytochrome c in HL-60 cells. Nagase, M., Alam, M.M., Tsushima, A., Yoshizawa, T., Sakato, N. Biosci. Biotechnol. Biochem. (2001) [Pubmed]
  22. Curcumin induces caspase-3-dependent apoptotic pathway but inhibits DNA fragmentation factor 40/caspase-activated DNase endonuclease in human Jurkat cells. Sikora, E., Bielak-Zmijewska, A., Magalska, A., Piwocka, K., Mosieniak, G., Kalinowska, M., Widlak, P., Cymerman, I.A., Bujnicki, J.M. Mol. Cancer Ther. (2006) [Pubmed]
  23. Caspase-3 cleavage and nuclear localization of caspase-activated DNase in human temporal lobe epilepsy. Schindler, C.K., Pearson, E.G., Bonner, H.P., So, N.K., Simon, R.P., Prehn, J.H., Henshall, D.C. J. Cereb. Blood Flow Metab. (2006) [Pubmed]
  24. Oligonucleosomal DNA fragmentation in MCF-7 cells undergoing palmitate-induced apoptosis. Semenov, D.V., Aronov, P.A., Kuligina, E.V., Potapenko, M.O., Richter, V.A. Biochemistry Mosc. (2003) [Pubmed]
  25. Contrasting nuclear dynamics of the caspase-activated DNase (CAD) in dividing and apoptotic cells. Lechardeur, D., Xu, M., Lukacs, G.L. J. Cell Biol. (2004) [Pubmed]
  26. Merbarone induces activation of caspase-activated DNase and excision of chromosomal DNA loops from the nuclear matrix. Otake, Y., Mims, A., Fernandes, D.J. Mol. Pharmacol. (2006) [Pubmed]
  27. DNA topoisomerase IIalpha interacts with CAD nuclease and is involved in chromatin condensation during apoptotic execution. Durrieu, F., Samejima, K., Fortune, J.M., Kandels-Lewis, S., Osheroff, N., Earnshaw, W.C. Curr. Biol. (2000) [Pubmed]
  28. Identification of the nuclear factor HMG2 as an activator for DFF nuclease activity. Toh, S.Y., Wang, X., Li, P. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  29. Erythropoietin activates caspase-3 and downregulates CAD during erythroid differentiation in TF-1 cells - a protection mechanism against DNA fragmentation. Lui, J.C., Kong, S.K. FEBS Lett. (2006) [Pubmed]
  30. Modulation of DNA fragmentation factor 40 nuclease activity by poly(ADP-ribose) polymerase-1. West, J.D., Ji, C., Marnett, L.J. J. Biol. Chem. (2005) [Pubmed]
  31. Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G. Widlak, P., Garrard, W.T. J. Cell. Biochem. (2005) [Pubmed]
  32. Identification of two contiguous minimally deleted regions on chromosome 1p36.31-p36.32 in oligodendroglial tumours. Dong, Z., Pang, J.S., Ng, M.H., Poon, W.S., Zhou, L., Ng, H.K. Br. J. Cancer (2004) [Pubmed]
  33. The contribution of apoptosis-inducing factor, caspase-activated DNase, and inhibitor of caspase-activated DNase to the nuclear phenotype and DNA degradation during apoptosis. Yuste, V.J., Sánchez-López, I., Solé, C., Moubarak, R.S., Bayascas, J.R., Dolcet, X., Encinas, M., Susin, S.A., Comella, J.X. J. Biol. Chem. (2005) [Pubmed]
  34. Molecular cloning and characterization of human caspase-activated DNase. Mukae, N., Enari, M., Sakahira, H., Fukuda, Y., Inazawa, J., Toh, H., Nagata, S. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  35. Generation of aberrant forms of DFF40 concurrent with caspase-3 activation during acute and chronic liver injury in rats. Xiang, Y., Johnson, E.A., Zhang, C., Huang, G., Hayes, R.L., Wang, K.K., Svetlov, S.I. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
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