Disease relevance of pufL

• Evolutionary distance of pufL and pufM, as assessed by substitution frequency analysis, confirms the closeness of the two Rhodobacter species, the other two species being equidistant from one another [1].
• In Rhodospirillum rubrum, pufL, and pufM, the structural genes coding for the photoreaction center L and M polypeptides, are comprised respectively of 831 and 921 nucleotides [1].
• The nucleotide sequences of the pufL and -M of Acidiphilium cryptum, Acidiphilium multivorum, and Acidiphilium organovorum were also determined [2].

High impact information on pufL

• It has been demonstrated before that a photosynthetically incompetent pufLMX deletion strain was not complemented by a plasmid-borne truncated puf operon version lacking only pufX, although expression of the pufL and pufM gene products was restored [3].
• The 3' termini of the small transcripts are located in the intercistronic region between pufA and pufL, downstream of another region of dyad symmetry [4].
• Interspecies evolutionary distance is greater for pufL than for pufM [1].
• Five site-directed mutants were engineered to substitute phenylalanine, serine, leucine, methionine, and glycine for tyrosine residue 162 of the pufL gene in Rhodobacter (R.) sphaeroides [5].
• Tyrosine 162 of the photosynthetic reaction center L-subunit plays a critical role in the cytochrome c2 mediated rereduction of the photooxidized bacteriochlorophyll dimer in Rhodobacter sphaeroides. 2. Quantitative kinetic analysis [6].

Biological context of pufL

• A new mutation in the pufL gene responsible for the terbutryn resistance phenotype in Rubrivivax gelatinosus [7].
• Sequence analysis of the mutant revealed a new mutation in the pufL gene coding for the L subunit of the reaction centre (RC) at codon 192 leading to an amino-acid substitution from Gly in the wild type to Asp in the mutant [7].

Analytical, diagnostic and therapeutic context of pufL

• An S-1 nuclease protection assay revealed that the 0.5-kb transcript encoded the LHI-alpha and LHI-beta subunits (pufBA), the 1.2-kb transcript encoded puf-BA and part of pufL, and the 1.9-kb transcript encoded pufBAL and part of pufM [8].
• Tyrosine 162 of the photosynthetic reaction center L-subunit plays a critical role in the cytochrome c2 mediated rereduction of the photooxidized bacteriochlorophyll dimer in Rhodobacter sphaeroides. 1. Site-directed mutagenesis and initial characterization [5].

References