High impact information on KCNA10

• In situ hybridization experiments confirmed the immunocytochemical studies and revealed KCNA10 expression in human proximal tubular cells, glomerular and vascular endothelial cells, and also in vascular smooth muscle cells [1].
• Expression of KCNA10, a voltage-gated K channel, in glomerular endothelium and at the apical membrane of the renal proximal tubule [1].
• The data suggest that KCNA10 may facilitate proximal tubular sodium absorption by stabilizing cell membrane voltage [1].
• Interacting clones were then rescreened in a functional assay by coinjection with KCNA10 in Xenopus oocytes [2].
• Because one distinguishing feature of KCNA10 is a putative cyclic nucleotide-binding domain located at the COOH terminus, the entire COOH-terminal region was used to probe a human cardiac cDNA library using the yeast two-hybrid system [2].

Biological context of KCNA10

• Given its distribution in renal blood vessels and heart, we speculate that KCNA10 may be involved in regulating the tone of renal vascular smooth muscle and may also participate in the cardiac action potential [3].
• These data indicate human KCNA10 maps to 1p13.1 and resides within the genetic interval defined by microsatellite loci D1S2809 and D1S2726 [4].
• Fluorescence in situ hybridization indicates that human KCNA10 maps to chromosome 1 at p13.1-->p22 [4].
• A novel Shaker-related potassium channel subunit termed Tsha3 that is widely expressed in the CNS of trout was PCR-cloned and sequenced: its deduced amino acid sequence showed an extended N-terminal domain with a high proportion of negatively charged residues and possessed highest similarity with KCNA10, a human epithelial potassium channel [5].

Other interactions of KCNA10

• RECENT FINDINGS: Several types of voltage-gated K+ (Kv) channel, such as KCNQ1, KCNA10 and Kv1.3, are highly expressed at the apical membrane of proximal tubules and distal tubules [6].

Analytical, diagnostic and therapeutic context of KCNA10

• KCNA10 is a cyclic nucleotide-gated, voltage-activated K channel that is detected in kidney, heart, and aorta by Northern blot and postulated to participate in renal K metabolism and to regulate vascular tone [1].

References