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Gene Review:

BFRF3 - capsid protein VP26

Human herpesvirus 4

Serio, T.R. et al., Lin, S.F. et al., Shedd, D. et al., van Grunsven, W.M. et al., Serio, T.R. et al.

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Disease relevance of BFRF3

Overexpressed KSHV sVCA purified from E. coli and used as an antigen in immunoblot screening assay did not cross-react with EBV BFRF3 [1].
Here we show that the 21-kDa component of the viral capsid antigen consists of products of two EBV genes, BFRF3 and BLRF2 [2].
The cDNA specified a 170-amino-acid peptide with homology to small viral capsid proteins encoded by the BFRF3 gene of Epstein-Barr virus and the ORF65 gene of herpesvirus saimiri [1].
Antibodies to the BFRF3 component of VCA became detectable approximately 2 months after onset of infectious mononucleosis [3].

High impact information on BFRF3

Late gene expression from the Epstein-Barr virus BcLF1 and BFRF3 promoters does not require DNA replication in cis [4].
In cells in which EBV was lytically replicating, BFRF3 protein was coimmunoprecipitated together with ZEBRA by a rabbit antiserum directed against amino acids 197 to 245 of BZLF1 [2].
Sequencing of the inserts of positive plaques showed that VCA-p18 and VCA-p40 are encoded within open reading frames (ORFs) BFRF3 and BdRF1, respectively [5].
Kinetics of the antibody response to BFRF3 were identical using supershift or immunoblotting assays [3].

Other interactions of BFRF3

The rabbit antiserum to amino acids 197 to 245 of BZLF1 was found to detect the same epitope at the carboxy end of BFRF3 as was recognized by rabbit antiserum to BFRF3 itself [2].

References

Identification, expression, and immunogenicity of Kaposi's sarcoma-associated herpesvirus-encoded small viral capsid antigen. Lin, S.F., Sun, R., Heston, L., Gradoville, L., Shedd, D., Haglund, K., Rigsby, M., Miller, G. J. Virol. (1997) [Pubmed]
Two 21-kilodalton components of the Epstein-Barr virus capsid antigen complex and their relationship to ZEBRA-associated protein p21 (ZAP21). Serio, T.R., Angeloni, A., Kolman, J.L., Gradoville, L., Sun, R., Katz, D.A., Van Grunsven, W., Middeldorp, J., Miller, G. J. Virol. (1996) [Pubmed]
Detection of human serum antibodies to the BFRF3 Epstein-Barr virus capsid component by means of a DNA-binding assay. Shedd, D., Angeloni, A., Niederman, J., Miller, G. J. Infect. Dis. (1995) [Pubmed]
Late gene expression from the Epstein-Barr virus BcLF1 and BFRF3 promoters does not require DNA replication in cis. Serio, T.R., Kolman, J.L., Miller, G. J. Virol. (1997) [Pubmed]
Gene mapping and expression of two immunodominant Epstein-Barr virus capsid proteins. van Grunsven, W.M., van Heerde, E.C., de Haard, H.J., Spaan, W.J., Middeldorp, J.M. J. Virol. (1993) [Pubmed]

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