The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

int  -  similar to GenBank Accession Number AAR05703

Escherichia coli

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of int

 

High impact information on int

  • This region also contains an open reading frame homologous to one present in Tn1696 (member of the Tn21 family) which encodes a site-specific integrase [5].
  • To facilitate the study of the attB site, the int and xis genes, expressed from an inducible promoter, and attP from pSAM2 were cloned on plasmids in Escherichia coil [6].
  • In this study, we show that expression of the natural int gene may also be modulated by rare arginine codon usage, and we explore this mechanism [1].
  • Both sib+ and sib- deletions were sequenced, and it was concluded from this and other work that a dyad symmetry present in the b region, 270 base-pairs from int, was necessary for retroregulation [2].
  • The lambda int gene product, integrase, recombines phage and bacterial DNA at a specific site during the integration step of lysogeny [2].
 

Biological context of int

  • Sequence analysis revealed the presence of an IS1 element between the int gene and the afa-3 gene cluster [7].
  • Plasmids containing the site-specific att/int functions of pMLP1 can be used to integrate genes into the chromosome [3].
  • DNAs homologous to bacteriophage int and attP were located upstream of gtrA(IV), suggesting that this region of the NCTC 8296 genome may have originated from a bacteriophage; however, a serotype-converting phage could not be induced from this strain nor from other strains used in this study [8].
 

Other interactions of int

  • The authors report here that phage Phi42 encodes a restriction-modification (R-M) system, termed Sau42I, adjacent to and in the same orientation to the phage integrase gene int [9].

References

  1. Modulation of lambda integrase synthesis by rare arginine tRNA. Zahn, K., Landy, A. Mol. Microbiol. (1996) [Pubmed]
  2. Deletion analysis of the retroregulatory site for the lambda int gene. Court, D., Huang, T.F., Oppenheim, A.B. J. Mol. Biol. (1983) [Pubmed]
  3. Development of the Micromonospora carbonacea var. africana ATCC 39149 bacteriophage pMLP1 integrase for site-specific integration in Micromonospora spp. Alexander, D.C., Devlin, D.J., Hewitt, D.D., Horan, A.C., Hosted, T.J. Microbiology (Reading, Engl.) (2003) [Pubmed]
  4. Phage TP901-1 site-specific integrase functions in human cells. Stoll, S.M., Ginsburg, D.S., Calos, M.P. J. Bacteriol. (2002) [Pubmed]
  5. Transposition of an antibiotic resistance element in mycobacteria. Martin, C., Timm, J., Rauzier, J., Gomez-Lus, R., Davies, J., Gicquel, B. Nature (1990) [Pubmed]
  6. Structure of the chromosomal insertion site for pSAM2: functional analysis in Escherichia coli. Raynal, A., Tuphile, K., Gerbaud, C., Luther, T., Guérineau, M., Pernodet, J.L. Mol. Microbiol. (1998) [Pubmed]
  7. Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster and its translocation via flanking IS1 insertion sequences. Garcia, M.I., Labigne, A., Le Bouguenec, C. J. Bacteriol. (1994) [Pubmed]
  8. Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296. Adams, M.M., Allison, G.E., Verma, N.K. Microbiology (Reading, Engl.) (2001) [Pubmed]
  9. Sau42I, a BcgI-like restriction-modification system encoded by the Staphylococcus aureus quadruple-converting phage Phi42. Dempsey, R.M., Carroll, D., Kong, H., Higgins, L., Keane, C.T., Coleman, D.C. Microbiology (Reading, Engl.) (2005) [Pubmed]
 
WikiGenes - Universities