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Gene Review

rnc  -  ribonuclease III

Escherichia coli O157:H7 str. Sakai

 
 
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Disease relevance of rncS

 

Psychiatry related information on rncS

  • However, low level activity is observed at extended reaction times and high enzyme concentrations, with an estimated catalytic efficiency approximately 15 000-fold lower than that of RNase III [6].
 

High impact information on rncS

 

Chemical compound and disease context of rncS

 

Biological context of rncS

 

Anatomical context of rncS

  • These observations establish the presence of a RNase III homolog in eukaryotic cells [20].
  • We find that these methods, stepwise chromatography on Whatman CF11-cellulose; digestion with Escherichia coli RNase III; and specific inhibition of globin synthesis in vitro in rabbit reticulocyte lysates, are able to distinguish between stable double-stranded RNA and single-stranded RNA in the expected manner [21].
  • As observed in an RNase III-deficient strain, processing of the IVS was not required for the production of functional ribosomes [22].
  • In an RNase III-deficient strain of E. coli 23S pre-rRNA accumulates unprocessed in 50S ribosomes and in polysomes [23].
  • Transient rescue experiments in HeLa cells demonstrated that the cleavage function of the rnc+ gene was necessary for full rescue of vp1080 [24].
 

Associations of rncS with chemical compounds

  • Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants [10].
  • Furthermore, RNase III cleaved the phosphorothioate internucleotide bond with 5' polarity [14].
  • Species C3 is also one of the 5' termini of 23S rRNA generated in vitro from larger precursors by the action of purified RNase III [25].
  • Specific overexpression and phosphorylation was used to locate the RNase III polypeptide in the standard two-dimensional gel pattern, and to confirm that serine is the phosphate-accepting amino acid [26].
  • For RNase III, a Mn(2+) concentration of 1 mM provides optimal activity, while concentrations >5 mM are inhibitory [6].
 

Physical interactions of rncS

  • Multicopy expression of rnc70 could suppress a lethal phenotype of the wild-type rnc allele in a certain genetic background; it could also inhibit the RNase III-mediated activation of lambdaN gene translation by competing for the RNA-binding site of the wild-type endonuclease [27].
 

Regulatory relationships of rncS

 

Other interactions of rncS

  • These results suggest that polynucleotide phosphorylase requires RNase III cleavages to autoregulate the translation of its message [29].
  • Some of these 3' extremities result from endonucleolytic cleavages by RNase E and RNase III and from exonucleolytic degradation [17].
  • A 240-nucleotide segment encompassing the 16S 3' end contains another RNase III site and the point of presumed RNase P scission at the 5' end of tRNA1Ile, the first tRNA appearing in the 16-23S spacer region of rrnD and rrnX [30].
  • RNase III, a double-stranded RNA-specific endonuclease, is proposed to be one of Escherichia coli's global regulators because of its ability to affect the expression of a large number of unrelated genes by influencing post-transcriptional control of mRNA stability or mRNA translational efficiency [27].
  • Statistical models, hidden Markov models (HMMs), were created for the RNase HII, III, II and PH and D families as well as a double-stranded RNA binding domain present in RNase III [31].
 

Analytical, diagnostic and therapeutic context of rncS

  • Northern blot, primer extension and S1 analyses indicated that S-CopA did not form a complete duplex with CopT in vivo since bands corresponding to RNase III cleavage products were missing [32].
  • Direct deproteinization of the particle yielded 30S RNA, while deproteinization after treatment with a crude RNase III preparation yielded products similar to 23S and 16S RNA [33].
  • Molecular cloning of the gene for the RNA-processing enzyme RNase III of Escherichia coli [12].
  • In an RNase III-deficient mutant of Escherichia coli, all 23 S ribosomal RNA in ribosomes is present in an unprocessed form with a double-stranded stem at the base of the molecule stable enough to be detected by electron microscopy under conditions where all other secondary structure is denatured [34].
  • The RNA processing functions, T4 RNA ligase, T4 polynucleotide kinase, and the host prr gene product appear not to be essential for exon ligation; neither are the host endoribonucleases RNase III, RNase P and RNase E required for intron excision [35].

References

  1. Escherichia coli ribonuclease III cleavage sites. Robertson, H.D. Cell (1982) [Pubmed]
  2. Processing of mRNA by ribonuclease III regulates expression of gene 1.2 of bacteriophage T7. Saito, H., Richardson, C.C. Cell (1981) [Pubmed]
  3. Cleavage of adenovirus messenger RNA and of 28S and 18S ribosomal RNA by RNase III. Westphal, H., Crouch, R.J. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  4. Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor. Oguro, A., Kakeshita, H., Nakamura, K., Yamane, K., Wang, W., Bechhofer, D.H. J. Biol. Chem. (1998) [Pubmed]
  5. The absB gene encodes a double strand-specific endoribonuclease that cleaves the read-through transcript of the rpsO-pnp operon in Streptomyces coelicolor. Chang, S.A., Bralley, P., Jones, G.H. J. Biol. Chem. (2005) [Pubmed]
  6. Mechanism of action of Escherichia coli ribonuclease III. Stringent chemical requirement for the glutamic acid 117 side chain and Mn2+ rescue of the Glu117Asp mutant. Sun, W., Nicholson, A.W. Biochemistry (2001) [Pubmed]
  7. Role of plasmid-coded RNA and ribonuclease III in plasmid DNA replication. Conrad, S.E., Campbell, J.L. Cell (1979) [Pubmed]
  8. OOP RNA, produced from multicopy plasmids, inhibits lambda cII gene expression through an RNase III-dependent mechanism. Krinke, L., Wulff, D.L. Genes Dev. (1987) [Pubmed]
  9. Thirty-three years later, a glimpse at the ribonuclease III active site. Zamore, P.D. Mol. Cell (2001) [Pubmed]
  10. Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants. Li, H., Nicholson, A.W. EMBO J. (1996) [Pubmed]
  11. Ribonuclease III cleavage of bacteriophage T3RNA polymerase transcripts to late T3 mRNAs. Majumder, H.K., Bishayee, S., Chakraborty, P.R., Maitra, U. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  12. Molecular cloning of the gene for the RNA-processing enzyme RNase III of Escherichia coli. Watson, N., Apirion, D. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  13. The 30 S ribosomal precursor RNA from Escherichia coli. A primary transcript containing 23 S, 16 S, and 5 S sequences. Ginsburg, D., Steitz, J.A. J. Biol. Chem. (1975) [Pubmed]
  14. Accurate in vitro cleavage by RNase III of phosphorothioate-substituted RNA processing signals in bacteriophage T7 early mRNA. Nicholson, A.W., Niebling, K.R., McOsker, P.L., Robertson, H.D. Nucleic Acids Res. (1988) [Pubmed]
  15. Structure of the dsRNA binding domain of E. coli RNase III. Kharrat, A., Macias, M.J., Gibson, T.J., Nilges, M., Pastore, A. EMBO J. (1995) [Pubmed]
  16. The global regulator RNase III modulates translation repression by the transcription elongation factor N. Wilson, H.R., Yu, D., Peters, H.K., Zhou, J.G., Court, D.L. EMBO J. (2002) [Pubmed]
  17. The rpsO mRNA of Escherichia coli is polyadenylated at multiple sites resulting from endonucleolytic processing and exonucleolytic degradation. Haugel-Nielsen, J., Hajnsdorf, E., Regnier, P. EMBO J. (1996) [Pubmed]
  18. NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. Bycroft, M., Grünert, S., Murzin, A.G., Proctor, M., St Johnston, D. EMBO J. (1995) [Pubmed]
  19. A nucleotide sequence from a ribonuclease III processing site in bacteriophage T7 RNA. Robertson, H.D., Dickson, E., Dunn, J.J. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  20. S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase. Iino, Y., Sugimoto, A., Yamamoto, M. EMBO J. (1991) [Pubmed]
  21. Sensitive methods for the detection and characterization of double helical ribonucleic acid. Robertson, H.D., Hunter, T. J. Biol. Chem. (1975) [Pubmed]
  22. Functional Escherichia coli 23S rRNAs containing processed and unprocessed intervening sequences from Salmonella typhimurium. Gregory, S.T., O'Connor, M., Dahlberg, A.E. Nucleic Acids Res. (1996) [Pubmed]
  23. Ordered processing of Escherichia coli 23S rRNA in vitro. Sirdeshmukh, R., Schlessinger, D. Nucleic Acids Res. (1985) [Pubmed]
  24. Complementation of deletion of the vaccinia virus E3L gene by the Escherichia coli RNase III gene. Shors, T., Jacobs, B.L. Virology (1997) [Pubmed]
  25. Escherichia coli 23S ribosomal RNA truncated at its 5' terminus. Sirdeshmukh, R., Krych, M., Schlessinger, D. Nucleic Acids Res. (1985) [Pubmed]
  26. Phosphorylation of elongation factor G and ribosomal protein S6 in bacteriophage T7-infected Escherichia coli. Robertson, E.S., Aggison, L.A., Nicholson, A.W. Mol. Microbiol. (1994) [Pubmed]
  27. Genetic uncoupling of the dsRNA-binding and RNA cleavage activities of the Escherichia coli endoribonuclease RNase III--the effect of dsRNA binding on gene expression. Dasgupta, S., Fernandez, L., Kameyama, L., Inada, T., Nakamura, Y., Pappas, A., Court, D.L. Mol. Microbiol. (1998) [Pubmed]
  28. Polynucleotide phosphorylase of Escherichia coli induces the degradation of its RNase III processed messenger by preventing its translation. Robert-Le Meur, M., Portier, C. Nucleic Acids Res. (1994) [Pubmed]
  29. E.coli polynucleotide phosphorylase expression is autoregulated through an RNase III-dependent mechanism. Robert-Le Meur, M., Portier, C. EMBO J. (1992) [Pubmed]
  30. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA. Young, R.A., Steitz, J.A. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  31. Comparative sequence analysis of ribonucleases HII, III, II PH and D. Mian, I.S. Nucleic Acids Res. (1997) [Pubmed]
  32. Replication control in plasmid R1: duplex formation between the antisense RNA, CopA, and its target, CopT, is not required for inhibition of RepA synthesis. Wagner, E.G., Blomberg, P., Nordström, K. EMBO J. (1992) [Pubmed]
  33. A ribonucleoprotein precursor of both the 30S and 50S ribosomal sunbunits of Escherichia coli. Duncan, M.J., Gorini, L. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  34. Why is processing of 23 S ribosomal RNA in Escherichia coli not obligate for its function? Sirdeshmukh, R., Schlessinger, D. J. Mol. Biol. (1985) [Pubmed]
  35. RNA splicing and in vivo expression of the intron-containing td gene of bacteriophage T4. Belfort, M., Pedersen-Lane, J., Ehrenman, K., Chu, F.K., Maley, G.F., Maley, F., McPheeters, D.S., Gold, L. Gene (1986) [Pubmed]
 
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