Disease relevance of ARHGAP6

• Expression in Escherichia coli of human ARHGAP6 gene and purification of His-tagged recombinant protein [1].

High impact information on ARHGAP6

• Functional analysis of ARHGAP6, a novel GTPase-activating protein for RhoA [2].
• 3. Our laboratory isolated a novel rho GTPase-activating protein (rhoGAP) gene named ARHGAP6 from the MLS region [2].
• ARHGAP6 contains 14 exons encoding a 974 amino acid protein with three putative SH3-binding domains [2].
• Transfected mammalian cells expressing ARHGAP6 lose their actin stress fibers, retract from the growth surface and extend thin, branching processes resembling filopodia [2].
• The ARHGAP6 protein co-localizes with actin filaments through an N-terminal domain and recruits F-actin into the growing processes [2].

Biological context of ARHGAP6

• The optimal cell density for expression of soluble 6xHis-ARHGAP6 protein was at A(600) about 0 [1].
• Molecular cytogenetic data suggest that the Xp22.3 inversion breakpoint is located in intron 1 of ARHGAP6, the gene encoding the Rho GTPase activating protein 6 [3].

Anatomical context of ARHGAP6

• We pursued two approaches to study the function of ARHGAP6 and its role in the pathogenesis of MLS: gene targeting of the rhoGAP domain in mouse embryonic stem cells and in vitro expression studies [2].
• The predicted protein sequence of the gene, named ARHGAP6, contains homology to the GTPase-activating (GAP) domain of the rhoGAP family of proteins, which has been implicated in the regulation of actin polymerization at the plasma membrane in several cellular processes [4].

Other interactions of ARHGAP6

• 3. End-sequencing and database analysis revealed a YAC insert of at least 416 kb containing the genes HCCS and AMELX, and exons 2-16 of ARHGAP6 [3].

References