High impact information on LMNB1

• A close juxtaposition of p58/lamin B-containing vesicles and chromosomes is already detectable in metaphase; however, p58 and lamin reassembly proceeds slowly and is completed in late telophase--G1 [1].
• Current models proposing functional specializations of mammalian lamin subtypes are in fact largely based on the observation that during mitosis mammalian lamin B remains associated with membrane vesicles, whereas lamins A and C become freely soluble [2].
• From these results we conclude that both chicken lamins B1 and B2 may functionally resemble mammalian lamin B. Interestingly, immunolabeling of mitotic cells revealed an association of lamin B2 with extended membrane cisternae that resembled elements of the endoplasmic reticulum [2].
• During embryonic development, lamin A became increasingly prominent, whereas the amounts of lamin B1 decreased in many tissues [3].
• The lamin B receptor (LBR) is an integral protein of the inner nuclear membrane that interacts with lamin B in vitro [4].

Anatomical context of LMNB1

• Oligomeric structure of the major nuclear envelope protein lamin B [5].
• These proteins migrate to characteristic positions on two-dimensional gels, lamin B being more acidic than lamins A and C. Here, we show that the composition of the nuclear lamina in avian and mammalian cells is more complex than previously assumed [6].
• We had identified earlier a germ cell-specific lamin of 60 kDa in rat which is related to somatic lamin B. This polypeptide was shown to be the only major component organizing the lamina structure of round spermatids [7].

References