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Gene Review

Lmnb1  -  lamin B1

Rattus norvegicus

Synonyms: Lamin-B1
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Disease relevance of Lmnb1

  • The DNA region established stable associations with nuclear lamin B (67 kDa, pI 5.7) in the controls, and with lamins A (69 kDa, pI 7.0), B, isoforms of lamin C (62 kDa, pI 6.55-6.95), and a 55-kDa (pI 5.9) polypeptide during the acute phase response [1].

High impact information on Lmnb1


Biological context of Lmnb1


Anatomical context of Lmnb1

  • These findings would represent an extension of the model proposed by Georgatos and Blobel (Georgatos, S. D., and G. Blobel. 1987a. J. Cell Biol. 105:105-115) in which type III intermediate size filaments are vectorially inserted to plasma and nuclear membranes by ankyrin and lamin B, respectively [5].
  • In this work, we show that polyclonal antibodies specific for lamin B recognizes a component of the plasma membrane of Torpedo electrocyte [5].
  • Approximately 50 to 60% of the lamin B present in matrix-envelope preparations was found in these insoluble membranes while a smaller amount of lamin A and even less of lamin C resisted complete extraction [10].
  • Disappearance of NUP153 and lamin B was coincident with onset of DNA fragmentation and clustering of nuclear pores [11].
  • The elevation of nuclear proteolytic activity was accompanied by site-specific degradation of nuclear mitotic apparatus protein and lamin B, two essential components of the nuclear matrix [9].

Associations of Lmnb1 with chemical compounds

  • After purification of the lamins from such urea extracts, a specific binding between isolated vimentin and lamin B, or a lamin A + B hetero-oligomer, was detected by affinity chromatography [12].
  • The location of lamin B as an intrinsic membrane protein was also established by photoaffinity labeling with the membrane-penetrating reagent azidopyrene [10].
  • Lithium pretreatment blocks glutamate-induced cytochrome c release and cleavage of lamin B1, a nuclear substrate for caspase-3 [13].
  • Cleavage of lamin B, which generated a fragment of 46 kDa consistent with the central rod domain of the protein, was also detected after 30 minutes of exposure to the steroid hormone [9].
  • The level of lamin B phosphorylation did not change as a result of the dexamethasone treatment and the lamina did not solubilize until the later stages of apoptosis [9].

Regulatory relationships of Lmnb1

  • Interleukin-1 beta induces posttranslational carboxymethylation and alterations in subnuclear distribution of lamin B in insulin-secreting RINm5F cells [14].

Other interactions of Lmnb1

  • Activation of intracellular caspases, manifest by proteolytic poly (ADP-ribose) polymerase (PARP) and lamin B cleavage, was maximal at 15 h after IR, concident with other indices of EC apoptosis, including oligonucleosomal DNA degradation, TUNEL immunostaining, and morphologic changes [15].
  • By other approaches (quantitative immunoprecipitation, rate zonal sedimentation, turbidometric assays) a substoichiometric lamin B-vimentin binding was determined under in vitro conditions [12].
  • A synthetic peptide, comprising the first 32 amino acid residues of lamins A and C, is able to fully compete with the intact molecules for binding to lamin B. Conversely, heterotypic A-C associations and homotypic A-A and C-C interactions appear significantly weaker than A/C-B binding and do not involve the lamin A and C amino-terminal domain [6].
  • Upon Te treatment, no degradation of the caspase substrates poly (ADP-ribose) polymerase and lamin B was detected by Western blots or immunocytochemistry, respectively [16].
  • With this in mind, we have used immunogold labeling to examine the topological associations of desmin cytoskeletal and lamin B nucleoskeletal intermediate filaments with various intracellular structures in mammalian cardiac myocytes [17].

Analytical, diagnostic and therapeutic context of Lmnb1

  • This conclusion was based on (a) transmission electron microscopy, (b) lipid analysis, (c) lamin B as an inner membrane-associated marker, and (d) the demonstration of phospholipid lateral mobility on outer membrane-depleted nuclei as a criteria for inner membrane integrity [18].
  • When hepatocytes were stimulated to divide by partial hepatectomy, only synthesis of lamin B was initiated [19].
  • For gel electrophoresis and Western blotting, we used antibodies to poly (ADP-ribose) polymerase (PARP), lamin B, and PKC-delta, as specific cleavage substrates of caspases [20].
  • The lamina structure of pre-meiotic spermatogonial nucleus contains both somatic lamin B and lamin(g) as evidenced by immunofluorescence studies with two differently fluorochrome labelled anti-lamin B and anti-lamin(g) antibodies [21].
  • In proliferating cells (tumors, regenerating liver, log phase cell culture) a polypeptide band of 150 kD and lamin B were conspicuous at the expense of lamins A and C [22].


  1. Regions of the haptoglobin 5' flanking gene sequence show different binding affinities to nuclear matrix proteins during the acute phase response. Poznanovic, G., Grujic, V., Ivanovic-Matic, S., Sevaljevic, L. J. Biochem. (1994) [Pubmed]
  2. Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Foisner, R., Gerace, L. Cell (1993) [Pubmed]
  3. Binding of matrix attachment regions to lamin B1. Ludérus, M.E., de Graaf, A., Mattia, E., den Blaauwen, J.L., Grande, M.A., de Jong, L., van Driel, R. Cell (1992) [Pubmed]
  4. Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope. Furukawa, K., Panté, N., Aebi, U., Gerace, L. EMBO J. (1995) [Pubmed]
  5. Presence of a protein immunologically related to lamin B in the postsynaptic membrane of Torpedo marmorata electrocyte. Cartaud, A., Courvalin, J.C., Ludosky, M.A., Cartaud, J. J. Cell Biol. (1989) [Pubmed]
  6. Heterotypic and homotypic associations between the nuclear lamins: site-specificity and control by phosphorylation. Georgatos, S.D., Stournaras, C., Blobel, G. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  7. Resistance of a variant ras-transformed cell line to phenotypic reversion by farnesyl transferase inhibitors. Prendergast, G.C., Davide, J.P., Lebowitz, P.F., Wechsler-Reya, R., Kohl, N.E. Cancer Res. (1996) [Pubmed]
  8. Protein kinase C-mediated interphase lamin B phosphorylation and solubilization. Collas, P., Thompson, L., Fields, A.P., Poccia, D.L., Courvalin, J.C. J. Biol. Chem. (1997) [Pubmed]
  9. Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes. Weaver, V.M., Carson, C.E., Walker, P.R., Chaly, N., Lach, B., Raymond, Y., Brown, D.L., Sikorska, M. J. Cell. Sci. (1996) [Pubmed]
  10. Lamin B from rat liver nuclei exists both as a lamina protein and as an intrinsic membrane protein. Lebel, S., Raymond, Y. J. Biol. Chem. (1984) [Pubmed]
  11. Sequential degradation of proteins from the nuclear envelope during apoptosis. Kihlmark, M., Imreh, G., Hallberg, E. J. Cell. Sci. (2001) [Pubmed]
  12. Lamin B constitutes an intermediate filament attachment site at the nuclear envelope. Georgatos, S.D., Blobel, G. J. Cell Biol. (1987) [Pubmed]
  13. Long term lithium treatment suppresses p53 and Bax expression but increases Bcl-2 expression. A prominent role in neuroprotection against excitotoxicity. Chen, R.W., Chuang, D.M. J. Biol. Chem. (1999) [Pubmed]
  14. Interleukin-1 beta induces posttranslational carboxymethylation and alterations in subnuclear distribution of lamin B in insulin-secreting RINm5F cells. Veluthakal, R., Amin, R., Kowluru, A. Am. J. Physiol., Cell Physiol. (2004) [Pubmed]
  15. Early molecular changes in irradiated aortic endothelium. Gajdusek, C., Onoda, K., London, S., Johnson, M., Morrison, R., Mayberg, M. J. Cell. Physiol. (2001) [Pubmed]
  16. Necrosis of schwann cells during tellurium-induced primary demyelination: DNA fragmentation, reorganization of splicing machinery, and formation of intranuclear rods of actin. Berciano, M.T., Fernandez, R., Pena, E., Calle, E., Villagra, N.T., Lafarga, M. J. Neuropathol. Exp. Neurol. (1999) [Pubmed]
  17. Trans-cellular desmin-lamin B intermediate filament network in cardiac myocytes. Lockard, V.G., Bloom, S. J. Mol. Cell. Cardiol. (1993) [Pubmed]
  18. Lateral diffusion in nuclear membranes. Schindler, M., Holland, J.F., Hogan, M. J. Cell Biol. (1985) [Pubmed]
  19. Expression of nuclear matrix proteins in rat liver tissue. Bibor-Hardy, V., LeMyre, A., Sakr, F., Bernard, M. Exp. Cell Res. (1991) [Pubmed]
  20. Expression of caspases and their substrates in the rat model of focal cerebral ischemia. Krupinski, J., Lopez, E., Marti, E., Ferrer, I. Neurobiol. Dis. (2000) [Pubmed]
  21. Behaviour of the germ cell specific lamin through mammalian spermatogenesis as probed with monoclonal antibodies. Manjula, K., Karande, A., Rao, M.R. Cell Struct. Funct. (1994) [Pubmed]
  22. Protein patterns of the nuclear matrix in differently proliferating and malignant cells. Kuzmina, S.N., Buldyaeva, T.V., Akopov, S.B., Zbarsky, I.B. Mol. Cell. Biochem. (1984) [Pubmed]
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