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Gene Review:

EMSP1 - enamel matrix serine proteinase 1

Sus scrofa

Fukae, M. et al., Hu, J.C. et al., Scully, J.L. et al.

Fukae, Tanabe, Nagano, Ando, Yamakoshi, Yamada, Simmer, Oida,

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High impact information on EMSP1

We conclude that odontoblasts deposit EMSP1 via their cell processes into the deepest enamel layer, which facilitates the hardening of this layer and contributes significantly to the functional properties of the EDJ [1].
Soft-tissue fractions, which included cells from the extension site of enamel formation (ESEF), secretory- and maturation-stage ameloblasts, and odontoblasts, were characterized histologically and by RT-PCR for their expression of enamelysin and EMSP1 [1].
A significant finding was that EMSP1, expressed by odontoblasts, concentrates in the HME, but is not detected in predentin or dentin [1].
Mouse EMSP1 shares 75% amino acid identity with pig EMSP1 and has three potential N-linked glycosylation sites, two of which are conserved in the pig homologue [2].
The expression of EMSP1 has never been investigated in another organism besides the pig, and EMSP1 expression in the enamel organ has never been specifically demonstrated in ameloblasts [2].

Biological context of EMSP1

We conclude that EMSP1 is expressed by pig and mouse ameloblasts during the early maturation stage of amelogenesis [2].

Associations of EMSP1 with chemical compounds

Recently we have cloned and characterized an mRNA encoding a tooth-specific serine proteinase designated enamel matrix serine proteinase 1 (EMSP1) [Simmer et al., JDR (1998) 77: 377] [3].

Analytical, diagnostic and therapeutic context of EMSP1

Western blot analysis shows that the polyclonal antibodies are specific for EMSP1 and do not cross-react with trypsin [2].
Stage-specific Northern blot analysis demonstrates this increase is accompanied by a parallel increase in the amount EMSP1 mRNA [3].

References

Odontoblasts enhance the maturation of enamel crystals by secreting EMSP1 at the enamel-dentin junction. Fukae, M., Tanabe, T., Nagano, T., Ando, H., Yamakoshi, Y., Yamada, M., Simmer, J.P., Oida, S. J. Dent. Res. (2002) [Pubmed]
Localization of EMSP1 expression during tooth formation and cloning of mouse cDNA. Hu, J.C., Ryu, O.H., Chen, J.J., Uchida, T., Wakida, K., Murakami, C., Jiang, H., Qian, Q., Zhang, C., Ottmers, V., Bartlett, J.D., Simmer, J.P. J. Dent. Res. (2000) [Pubmed]
Enamel matrix serine proteinase 1: stage-specific expression and molecular modeling. Scully, J.L., Bartlett, J.D., Chaparian, M.G., Fukae, M., Uchida, T., Xue, J., Hu, C.C., Simmer, J.P. Connect. Tissue Res. (1998) [Pubmed]

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