Disease relevance of OCT1

• However, the finding of an active basolateral organic cation transporter, together with the presence of gamma GT, dipeptidase and beta-lyase, makes this system especially interesting for testing all compounds that use this transporter or these enzymes in order to elicit toxicity [1].

High impact information on OCT1

• Primary structure and functional expression of the apical organic cation transporter from kidney epithelial LLC-PK1 cells [2].
• Since mepiperphenidol blocked the gentamicin-induced Ca2+ increases, the data suggest that aminoglycosides enter the cell via the organic cation transporter [3].
• Functional expression of the renal organic cation transporter and P-glycoprotein in Xenopus laevis oocytes [4].
• The results suggest that PQ may be transported by the same cation transporter as cimetidine and not TEA, indicating PQ uptake in the bPTC to be via a polyvalent organic cation transporter [5].
• The organic cation transporter should be involved in the basolateral uptake of creatinine [6].

Anatomical context of OCT1

• The Xenopus oocyte system provides a functional approach to further characterize the OCT [4].
• Using [14C]tetraethylammonium bromide ([14C]TEA) as a substrate, we tested several renal cell lines for a nucleoside-sensitive OCT. American opossum kidney proximal tubule cells (OK) express a cimetidine-sensitive and metabolic-dependent ability to efflux TEA [7].

Associations of OCT1 with chemical compounds

• Previous work (J. A. Nelson, J. F. Kuttesch, Jr., and B. H. Herbert. Biochemical Pharmacology 32: 2323-2327, 1983) indicated a role for the classic organic cation transporter (OCT) in the secretion of the dAdo analog, 2'-deoxytubercidin, by mouse kidney [7].

Other interactions of OCT1

• Previously we cloned membrane associated (M(r) 62000-67000) polypeptides from pig (pRS1), rabbit (rbRS1) and man (hRS1) which modified transport activities that were expressed in Xenopus laevis oocytes by the Na(+)-D-glucose cotransporter SGLT1 and/or the organic cation transporter OCT2 [8].

References