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Gene Review:

nahH - catechol-2, 3-dioxygenase NahH

Pseudomonas putida

Ghosal, D. et al., You, I.S. et al., Okuta, A. et al., Cerdan, P. et al.

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Disease relevance of nahH

The enzyme catechol 2,3-dioxygenase (C23O) encoded by the nahH gene of plasmid NAH7 converts catechol to alpha-hydroxymuconic epsilon-semialdehyde in Pseudomonas putida [1].
Clones of the nahH gene in Escherichia coli overproduce the protein product at least ten fold and the gene product was identified by polyacrylamide gel electrophoresis [1].

High impact information on nahH

The complete nucleotide sequence of the intergenic region spanning genes nahG and nahH has been determined and its biological role proposed [2].
To isolate this C23O, diverse C23O gene sequences were PCR amplified from DNA which had been isolated from mixed cultures of phenol-degrading bacteria and subcloned in the middle of a known C23O gene sequence (xylE or nahH) to construct a library of chimeric C23O genes [3].

Biological context of nahH

A small DNA segment (831 base pairs) disrupts the continuity of the known gene order nahG and nahH, the latter encoding catechol 2,3-dioxygenase [2].

Associations of nahH with chemical compounds

The substrate specificities of two catechol 2,3-dioxygenases, one encoded by xylE on the TOL plasmid pWW0 and the other encoded by nahH on the NAH7 plasmid, were investigated [4].

References

Nucleotide sequence and expression of gene nahH of plasmid NAH7 and homology with gene xylE of TOL pWWO. Ghosal, D., You, I.S., Gunsalus, I.C. Gene (1987) [Pubmed]
Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region. You, I.S., Ghosal, D., Gunsalus, I.C. Biochemistry (1991) [Pubmed]
Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methylcatechol. Okuta, A., Ohnishi, K., Harayama, S. Appl. Environ. Microbiol. (2004) [Pubmed]
Substrate specificity differences between two catechol 2,3-dioxygenases encoded by the TOL and NAH plasmids from Pseudomonas putida. Cerdan, P., Rekik, M., Harayama, S. Eur. J. Biochem. (1995) [Pubmed]

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