Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

relA - GDP/GTP pyrophosphokinase

Escherichia coli UTI89

Bugrysheva, J.V. et al., Gourse, R.L. et al., Imaizumi, A. et al., Nakanishi, N. et al., Kasai, K. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of relA

Identification and characterization of a second, inducible promoter of relA in Escherichia coli [1].

High impact information on relA

We find that rRNA synthesized from plasmids does exhibit a relA-dependent, stringent response [2].
The activation was dependent on relA and spoT, which encode enzymes for the synthesis and degradation of ppGpp, and on dksA, which encodes an RNA polymerase accessory protein required for the stringent response [3].
Subsequent in vitro assays using ribosomes isolated from wild-type and relA and relC mutant strains confirmed that (p)ppGpp is synthesized by ribosomes and that mutation of RelA or L11 blocks that activity [4].
In this study, we demonstrate that overexpression of the relA gene, encoding ppGpp synthetase, increases the accumulation of amino acids, such as glutamate and lysine, in amino-acid-overproducing strains of E. coli [5].

Chemical compound and disease context of relA

The global transcriptional regulator (p)ppGpp (guanosine-3'-diphosphate-5'-triphosphate and guanosine-3',5'-bisphosphate, collectively) produced by the relA and spoT genes in Escherichia coli allows bacteria to adapt to different environmental stresses [6].

Biological context of relA

The genome of Borrelia burgdorferi encodes a single chromosomal rel gene (BB0198) (B. burgdorferi rel [rel(Bbu)]) homologous to relA and spoT of E. coli [6].

References

Identification and characterization of a second, inducible promoter of relA in Escherichia coli. Nakagawa, A., Oshima, T., Mori, H. Genes Genet. Syst. (2006) [Pubmed]
Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivo. Gourse, R.L., Stark, M.J., Dahlberg, A.E. Cell (1983) [Pubmed]
ppGpp with DksA controls gene expression in the locus of enterocyte effacement (LEE) pathogenicity island of enterohaemorrhagic Escherichia coli through activation of two virulence regulatory genes. Nakanishi, N., Abe, H., Ogura, Y., Hayashi, T., Tashiro, K., Kuhara, S., Sugimoto, N., Tobe, T. Mol. Microbiol. (2006) [Pubmed]
Physiological Analysis of the Stringent Response Elicited in an Extreme Thermophilic Bacterium, Thermus thermophilus. Kasai, K., Nishizawa, T., Takahashi, K., Hosaka, T., Aoki, H., Ochi, K. J. Bacteriol. (2006) [Pubmed]
The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli. Imaizumi, A., Kojima, H., Matsui, K. J. Biotechnol. (2006) [Pubmed]
Borrelia burgdorferi rel is responsible for generation of guanosine-3'-diphosphate-5'-triphosphate and growth control. Bugrysheva, J.V., Bryksin, A.V., Godfrey, H.P., Cabello, F.C. Infect. Immun. (2005) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.