Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

ORF1 - ORF1 protein

Bos taurus

This record was discontinued.

Fleming, S.B. et al., Smiley, J.R. et al., Loeza-Lara, P.D. et al., Furth, J.J. et al., Nualart, F. et al., et al.

Nualart, Hein, Oliver, Asobayire, Dastjerdi, Bridger,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ORF1

ORF1 encoded the non-structural and contiguous capsid proteins [1].
Among caliciviruses, NB virus shows amino acid identities of 14.1 to 22.6% over the entire ORF-1 nonstructural-protein sequence with NLV, SLV, vesivirus, and lagovirus strains, while the overall sequence identity of the complete NB VP-1 with other caliciviruses is low, varying between 14.6 and 26.7% [2].
ORF-1 was transcribed more efficiently in recombinant virus-infected cells than in those infected with OV and analysis of the putative promoter, 5'-AAAATTGTAAATGTA, showed that it was similar to the 7.5-kDa early promoter of vaccinia virus [3].

High impact information on ORF1

Analysis of the 5' end of transcripts by S1 nuclease and primer extension showed that the transcriptional start points (tsp) of ORF-pp, ORF-1, and ORF-3 in the recombinant were identical or within four nucleotides of the tsps of the same ORFs in OV [3].
The 4.4-kb BamHI-E fragment of the orf virus (OV) genome contains three discrete open reading frames designated ORF-pp, ORF-1, and ORF-3, all of which are flanked by vaccinia virus-like early transcriptional control sequences [3].
Analyses of the long consensus sequence revealed an extended open reading frame (ORF-1) spanning from base 1,634 to 14,400 that encodes for a putative protein of 4,256 amino acids (approximately 450 kDa) [4].
Apart from the ORF1 NTPase region, their ORF1 regions had less than 90% identity to the genogroup III genotype 1 Bo/Jena/80/DE virus, confirming two genogroup III genotypes [5].
ORF1 encodes a 334-residue protein almost identical to the putative Rep proteins of previously sequenced S. aureus rolling-circle-replicating plasmids [6].

Biological context of ORF1

Two ORFs (ORF1 and ORF2) were identified, and their putative transcription initiation and Shine-Dalgarno sequence were localized [6].

Associations of ORF1 with chemical compounds

The deletion mutant, which contains 7 kb less BHV1 DNA than wt virus and lacks ORF1, US2, the genes for protein kinase, glycoprotein G, glycoprotein I, and most of glycoprotein E was still replication-competent [7].

Analytical, diagnostic and therapeutic context of ORF1

Northern blotting analysis of early RNA isolated from 143B-TK- cells infected with the recombinant virus showed that OV genes were transcribed and that the three transcripts of 0.70-(ORF-pp), 0.48- (ORF1), and 0.75-kb (ORF-3) were the same size as their counterparts in OV-infected cells [3].

References

Genomic characterization of the unclassified bovine enteric virus Newbury agent-1 (Newbury1) endorses a new genus in the family Caliciviridae. Oliver, S.L., Asobayire, E., Dastjerdi, A.M., Bridger, J.C. Virology (2006) [Pubmed]
Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus. Smiley, J.R., Chang, K.O., Hayes, J., Vinjé, J., Saif, L.J. J. Virol. (2002) [Pubmed]
In vivo recognition of orf virus early transcriptional promoters in a vaccinia virus recombinant. Fleming, S.B., Mercer, A.A., Fraser, K.M., Lyttle, D.J., Robinson, A.J. Virology (1992) [Pubmed]
Biosynthesis and molecular biology of the secretory proteins of the subcommissural organ. Nualart, F., Hein, S. Microsc. Res. Tech. (2001) [Pubmed]
Complete genomic characterization and antigenic relatedness of genogroup III, genotype 2 bovine noroviruses. Oliver, S.L., Asobayire, E., Charpilienne, A., Cohen, J., Bridger, J.C. Arch. Virol. (2007) [Pubmed]
pBMSa1, a plasmid from a dairy cow isolate of Staphylococcus aureus, encodes a lincomycin resistance determinant and replicates by the rolling-circle mechanism. Loeza-Lara, P.D., Soto-Huipe, M., Baizabal-Aguirre, V.M., Ochoa-Zarzosa, A., Valdez-Alarcón, J.J., Cano-Camacho, H., López-Meza, J.E. Plasmid (2004) [Pubmed]
Construction of a viable BHV1 mutant lacking most of the short unique region. Furth, J.J., Whitbeck, J.C., Lawrence, W.C., Bello, L.J. Arch. Virol. (1997) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.