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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

Gcn2  -  CG1609 gene product from transcript CG1609-RA

Drosophila melanogaster

Synonyms: CG1609, DGCN2, Dmel\CG1609, EIF2, GCN2, ...
 
 
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High impact information on Gcn2

  • The homologous N-terminus of Drosophila GCN2 interacted with yeast GCN1-GCN20 and had a dominant Gcn(-) phenotype, suggesting evolutionary conservation of this interaction [1].
  • Affinity-purified antibodies, raised against a synthetic peptide based on the predicted DGCN2 sequence, specifically immunoprecipitated an eIF-2alpha kinase activity and recognized an approximately 175 kDa phosphoprotein in Western blots of Drosophila embryo extracts [2].
  • It is particularly notable that DGCN2 possesses an amino acid sequence related to class II aminoacyl-tRNA synthetases, a unique feature of yeast GCN2 protein kinase [2].
  • During embryogenesis, DGCN2 mRNA is dynamically expressed in several tissues [2].
  • The identity of the Drosophila GCN2 (dGCN2) gene is supported by the unique combination of sequence encoding a protein kinase catalytic domain and a domain homologous to histidyl-tRNA synthetase and by the ability of dGCN2 to complement a deletion mutant of the yeast GCN2 gene [3].
 

Anatomical context of Gcn2

  • These are the hemin-regulated inhibitor (HRI), the interferon-inducible dsRNA-dependent kinase (PKR), the endoplasmic reticulum (ER)-resident kinase (PERK) and the GCN2 protein kinase [4].
 

Analytical, diagnostic and therapeutic context of Gcn2

  • We identified a C-terminal segment of GCN1 sufficient to bind the GI domain and used a novel dual bait two-hybrid method to identify mutations rendering GCN1 incapable of interacting with GCN2 [5].
  • We report the molecular cloning and characterization of DGCN2, a Drosophila eIF-2alpha kinase related to yeast GCN2 protein kinase [2].

References

  1. Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation. Garcia-Barrio, M., Dong, J., Ufano, S., Hinnebusch, A.G. EMBO J. (2000) [Pubmed]
  2. Cloning and characterization of a cDNA encoding a protein synthesis initiation factor-2alpha (eIF-2alpha) kinase from Drosophila melanogaster. Homology To yeast GCN2 protein kinase. Santoyo, J., Alcalde, J., Méndez, R., Pulido, D., de Haro, C. J. Biol. Chem. (1997) [Pubmed]
  3. Isolation of the gene encoding the Drosophila melanogaster homolog of the Saccharomyces cerevisiae GCN2 eIF-2alpha kinase. Olsen, D.S., Jordan, B., Chen, D., Wek, R.C., Cavener, D.R. Genetics (1998) [Pubmed]
  4. Functional characterization of Drosophila melanogaster PERK eukaryotic initiation factor 2alpha (eIF2alpha) kinase. Pomar, N., Berlanga, J.J., Campuzano, S., Hernández, G., Elías, M., de Haro, C. Eur. J. Biochem. (2003) [Pubmed]
  5. Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2. Kubota, H., Ota, K., Sakaki, Y., Ito, T. J. Biol. Chem. (2001) [Pubmed]
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