Disease relevance of MYCL2

• RNase protection analysis of a panel of human normal and tumor tissues has revealed that MYCL2 is expressed almost exclusively in human adult normal testis; much lower levels of transcript were detected in one human lung adenocarcinoma [1].

High impact information on MYCL2

• Antisense transcripts were found spanning regions of MYCL2 corresponding to all three exons of MYCL1 [1].
• No MYCL2 transcript was found in human testis RNA obtained from second trimester fetuses [1].
• No sizable open reading frame was seen for the MYCL2 antisense transcripts suggesting that they may represent either regulatory sequences or an intron of a gene encoded by the complementary strand [1].
• Northern blot analysis of poly(A)+ RNA from adult human normal testis with an antisense riboprobe revealed a transcript of approximately 4.8-kb, which is in agreement with the size predicted from the MYCL2 nucleotide sequence [1].
• We have characterized the expression of MYCL2, an intronless X-linked gene related to MYCL1 [1].

Biological context of MYCL2

• Mapping of the MYCL2 processed gene to Xq22-23 and identification of an additional L MYC-related sequence in Xq27.2 [2].
• This sequence is not the MYCL2 homology, previously mapped to the long arm of the X chromosome at q22-qter by Morton et al., as we located the MYCL2-processed gene in Xq22-23, using a panel containing a combination of hybrid DNA carrying different portions of the human X chromosome [2].
• RNase protection assays and the 5' RACE protocol (Rapid Amplification of cDNA Ends) were used to address the localization of the transcription start site of the MYCL2 sense transcript and different putative promoters and transcription regulatory elements have been identified [1].
• Mapping of MYCL2 by somatic cell hybrids places this sequence on the long arm of the X chromosome in bands q22----q28 [3].
• This processed gene (MYCL2) was isolated by cross-hybridization to an oligonucleotide probe synthesized from the C-MYC (MYC) sequence in a highly conserved region of the myc gene family [3].

Anatomical context of MYCL2

• This observation suggests a germ cell rather than somatic cell origin of the transcript and possible developmental regulation of MYCL2 [1].

Analytical, diagnostic and therapeutic context of MYCL2

• Sequence analysis of MYCL2 revealed an open reading frame of 1194 bp with no intervening sequences and strong homology to the recently published DNA sequence of MYCL1 [3].

References