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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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RpII18  -  RNA polymerase II 18kD subunit

Drosophila melanogaster

Synonyms: BcDNA:RH21608, CG1163, DNA-directed RNA polymerases I, II, and III subunit RPABC2, Dm6, Dmel\CG1163, ...
 
 
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High impact information on RpII18

  • We show that Pcf11 is directly involved in termination in Drosophila. dPcf11 is concentrated at the 3' end of the hsp70 gene in cells, and depletion of dPcf11 with RNAi causes Pol II to readthrough the normal region of termination. dPcf11 also localizes to most transcribed loci on polytene chromosomes [1].
  • Unlike the TAFs and Pol II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step [2].
  • Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites [2].
  • Rtt109 Is Required for Proper H3K56 Acetylation: A CHROMATIN MARK ASSOCIATED WITH THE ELONGATING RNA POLYMERASE II [3].
  • The multiprotein Mediator complex is a coactivator required for transcriptional activation of RNA polymerase II transcribed genes by DNA binding transcription factors [4].
 

Biological context of RpII18

  • Phosphorylation of the large RNA Polymerase II subunit C-terminal domain (CTD) is believed to be important in promoter clearance and for recruiting protein factors that function in messenger RNA synthesis and processing [5].
  • RESULTS: The collective information shows that the upstream region of early WSSV genes, containing a TATA box and an initiator, is similar to Drosophila RNA polymerase II core promoter sequences, suggesting utilization of the cellular transcription machinery for generating early transcripts [6].
  • The reliable recognition of eukaryotic RNA polymerase II core promoters, and the associated transcription start sites (TSSs) of genes, has been an ongoing challenge for computational biology [7].
 

Analytical, diagnostic and therapeutic context of RpII18

  • The chromatin immunoprecipitation assays revealed that HDI treatments induced the hyperacetylation of histone H3 at the promoter and the transcribing regions of hsp70 gene, increased the accessibility of heat-shock factor to target heat-shock element, and promoted the RNA polymerase II-mediated transcription [8].

References

  1. Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. Zhang, Z., Gilmour, D.S. Mol. Cell (2006) [Pubmed]
  2. Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock. Park, J.M., Werner, J., Kim, J.M., Lis, J.T., Kim, Y.J. Mol. Cell (2001) [Pubmed]
  3. Rtt109 Is Required for Proper H3K56 Acetylation: A CHROMATIN MARK ASSOCIATED WITH THE ELONGATING RNA POLYMERASE II. Schneider, J., Bajwa, P., Johnson, F.C., Bhaumik, S.R., Shilatifard, A. J. Biol. Chem. (2006) [Pubmed]
  4. A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. Sato, S., Tomomori-Sato, C., Banks, C.A., Parmely, T.J., Sorokina, I., Brower, C.S., Conaway, R.C., Conaway, J.W. J. Biol. Chem. (2003) [Pubmed]
  5. Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment. Eissenberg, J.C., Shilatifard, A., Dorokhov, N., Michener, D.E. Mol. Genet. Genomics (2007) [Pubmed]
  6. In silico identification of putative promoter motifs of White Spot Syndrome Virus. Marks, H., Ren, X.Y., Sandbrink, H., van Hulten, M.C., Vlak, J.M. BMC Bioinformatics (2006) [Pubmed]
  7. Identification of core promoter modules in Drosophila and their application in accurate transcription start site prediction. Ohler, U. Nucleic Acids Res. (2006) [Pubmed]
  8. Effects of histone deacetylase inhibitors on transcriptional regulation of the hsp70 gene in Drosophila. Zhao, Y.M., Chen, X., Sun, H., Yuan, Z.G., Ren, G.L., Li, X.X., Lu, J., Huang, B.Q. Cell Res. (2006) [Pubmed]
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