High impact information on PISD

• CRBL PSDC activity in aged membranes using agPS as substrate is lower than PSDC activity in adult membranes in the presence of adPS [1].
• A significant portion of brain phosphatidylethanolamine (PE) is synthesized by a pathway involving the mitochondrial enzyme phosphatidylserine decarboxylase (PSDC), in a process by which phosphatidylserine (PS) is transferred from the endoplasmic reticulum to mitochondria [1].
• The present study was carried out to determine PSDC activity in cerebral cortex (CC) and cerebellum (CRBL) mitochondrial fraction from adult (4-month-old) and aged (30-month-old) rats and to compare these activities with that found in liver [1].
• Age-related changes in central nervous system phosphatidylserine decarboxylase activity [1].
• The solubilization procedure of phosphatidylserine decarboxylase from mitochondrial membranes with Triton X-100 was elaborated [2].

Anatomical context of PISD

• To study the effect of 22:6n-3 content on the PSDC activity, PSs from different sources were prepared: rPS (from bovine retina, containing 36 mol % of 22:6n-3); adPS (from adult rat CC microsomal membranes, with 25 mole % 22:6n-3 content) and agPS (from aged rat CC microsomal membranes, with 21 mole % 22:6n-3 content) [1].

Associations of PISD with chemical compounds

• When this fraction was further separated by metrizamide density centrifugation, 90% of the phosphatidylserine decarboxylase activity was associated with the mitochondria [3].
• Effect of triton X-100 on the activity and solubilization of rat liver mitochondrial phosphatidylserine decarboxylase [2].

References