Disease relevance of RPS5

• The RPS5 disease resistance gene of Arabidopsis mediates recognition of Pseudomonas syringae strains that possess the avirulence gene avrPphB [1].

High impact information on RPS5

• A mutation within the leucine-rich repeat domain of the Arabidopsis disease resistance gene RPS5 partially suppresses multiple bacterial and downy mildew resistance genes [2].
• At a distance of 10 kb to either side of the locus, however, we find low levels of polymorphism and the absence of linkage disequilibrium between individual SNPs and RPS5 alleles [3].
• The island of "enhanced" variability extends several kilobases to either side of the RPS5 deletion junction, and these SNPs are in nearly complete linkage disequilibrium with the RPS5 insertion/deletion [3].
• From expression analyses we conclude that AtRPS5A is the most abundantly expressed RPS5 gene in Arabidopsis [4].
• We discuss several possible models for the roles of PBS1 and RPS5 in AvrPphB recognition [5].

Chemical compound and disease context of RPS5

• In Arabidopsis, resistance to Pseudomonas syringae strains expressing AvrPphB requires RPS5, a member of the class of R proteins that have a predicted nucleotide-binding site and leucine-rich repeats, and PBS1, a protein kinase [6].

Biological context of RPS5

• We present the analysis of an Arabidopsis promoter-trap line, in which a T-DNA insertion in an RPS5 gene (AtRPS5A) causes semi-dominant developmental phenotypes [4].
• Previous work has shown that RPS5 encodes a member of the nucleotide binding site-leucine rich repeat class of plant disease resistance genes [5].
• We conclude that loss of RPS5 was caused by a double-strand break and subsequent repair, and cannot be attributed to unequal crossing over between resistance gene homologs [7].
• DNA sequence from the latter ecotypes lacked a transposon footprint, suggesting that insertion of Tag2 occurred after the initial deletion of RPS5 [7].
• RPS5 was mapped to chromosome 1, between restriction fragment length polymorphism markers m241 and g3786 [8].

Regulatory relationships of RPS5

• Mutations in PBS1 completely blocked RPS5-mediated resistance, but had little to no effect on resistance specified by other disease resistance genes, suggesting that PBS1 facilitates recognition of the avrPphB protein [1].

Other interactions of RPS5

• By screening for loss of RPS5-specified resistance, we identified five pbs (avrPphB susceptible) mutants that represent three different genes [1].
• The Ler-0 ecotype contains RFL1, but lacks RPS5 [7].

Analytical, diagnostic and therapeutic context of RPS5

• Sequence analysis of the RPS5 deletion region in Ler-0 revealed the presence of an Ac-like transposable element, which we have designated Tag2 [7].

References