High impact information on CSM1

• The protein Csm1 is required for mono-orientation of sister kinetochores at meiosis I in budding yeast [1].
• Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex [2].
• Deletion of CSM1 causes incorrect spore formation and meiotic chromosome missegregation together with increased sensitivity of vegetative cells to benomyl and manganese [2].
• In a two-hybrid analysis with Csm1p as bait, we detected interactions with three members of the Mcm2-7 family of proteins involved in the initiation of DNA replication, and with Clf1p also implicated in replication [2].
• The Csm1p-Mcm3, Mcm5 and Mcm7p interactions were confirmed by co-immunoprecipitation [2].

Biological context of CSM1

• The level of Csm1p does not change throughout the cell cycle, but this protein is absent in mature spores [2].

Physical interactions of CSM1

• We describe here the identification of a protein complex, Csm1/Lrs4, that is essential for monoorientation of sister kinetochores in Saccharomyces cerevisiae [3].

References