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Gene Review

MCM3  -  MCM DNA helicase complex subunit MCM3

Saccharomyces cerevisiae S288c

Synonyms: DNA replication licensing factor MCM3, Minichromosome maintenance protein 3, SYGP-ORF23, YEL032W
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High impact information on MCM3

  • Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor [1].
  • Once inside the nucleus, a fraction of the MCM2 and MCM3 proteins becomes tightly associated with DNA [2].
  • We show further that the nuclear and subnuclear localizations of the MCM2 and MCM3 proteins are temporally regulated with respect to the cell cycle [2].
  • Here, we show that Cdc47p assembles into stable complexes with two other members of the MCM family, Cdc46p and Mcm3p [3].
  • MCM3 is an essential gene involved in the maintenance of minichromosomes in yeast cells [4].

Biological context of MCM3


Anatomical context of MCM3

  • The first requires the entry of a cytosolic 'loading factor' that is excluded by the nuclear membrane; subsequently, MCM3 can bind to chromatin in the presence or absence of a nuclear membrane, but only if the loading factor has gained access in the absence of the membrane [8].
  • BACKGROUND: GANP, carrying DNA-primase and MCM3-binding domains, is up-regulated in germinal centre B cells [9].

Associations of MCM3 with chemical compounds


Other interactions of MCM3

  • Furthermore, overproduction of Mcm3 accentuates the deleterious effect of the mcm2-1 mutation, whereas overproduction of Mcm2 partially complements the mcm3-1 mutation [5].
  • CDC45 also interacts genetically with ORC2, the gene encoding the second subunit of the origin recognition complex, ORC, and MCM3, another member of the MCM family [11].
  • We also identify a Crm1-dependent nuclear export signal (NES) adjacent to the Mcm3 NLS [12].
  • The mad2Delta mutation partially relieved the arrest responses of cells to mutations affecting the replication proteins Mcm3p and Pol1p [13].

Analytical, diagnostic and therapeutic context of MCM3


  1. Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor. Kubota, Y., Mimura, S., Nishimoto, S., Takisawa, H., Nojima, H. Cell (1995) [Pubmed]
  2. Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast. Yan, H., Merchant, A.M., Tye, B.K. Genes Dev. (1993) [Pubmed]
  3. Characterization of Cdc47p-minichromosome maintenance complexes in Saccharomyces cerevisiae: identification of Cdc45p as a subunit. Dalton, S., Hopwood, B. Mol. Cell. Biol. (1997) [Pubmed]
  4. The phenotype of the minichromosome maintenance mutant mcm3 is characteristic of mutants defective in DNA replication. Gibson, S.I., Surosky, R.T., Tye, B.K. Mol. Cell. Biol. (1990) [Pubmed]
  5. Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function. Yan, H., Gibson, S., Tye, B.K. Genes Dev. (1991) [Pubmed]
  6. Two mcm3 mutations affect different steps in the initiation of DNA replication. Lei, M., Cheng, I.H., Roberts, L.A., McAlear, M.A., Tye, B.K. J. Biol. Chem. (2002) [Pubmed]
  7. The possible involvement of replication-related proteins with a DEAD-box-like motif in cell-free DNA replication of Xenopus eggs. Someya, A., Shioda, M. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
  8. The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts. Madine, M.A., Khoo, C.Y., Mills, A.D., Musahl, C., Laskey, R.A. Curr. Biol. (1995) [Pubmed]
  9. MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR. Kono, Y., Maeda, K., Kuwahara, K., Yamamoto, H., Miyamoto, E., Yonezawa, K., Takagi, K., Sakaguchi, N. Genes Cells (2002) [Pubmed]
  10. Reconstitution of the Mcm2-7p heterohexamer, subunit arrangement, and ATP site architecture. Davey, M.J., Indiani, C., O'Donnell, M. J. Biol. Chem. (2003) [Pubmed]
  11. Identification of Cdc45p, an essential factor required for DNA replication. Hardy, C.F. Gene (1997) [Pubmed]
  12. CDK phosphorylation of a novel NLS-NES module distributed between two subunits of the Mcm2-7 complex prevents chromosomal rereplication. Liku, M.E., Nguyen, V.Q., Rosales, A.W., Irie, K., Li, J.J. Mol. Biol. Cell (2005) [Pubmed]
  13. Overlapping roles of the spindle assembly and DNA damage checkpoints in the cell-cycle response to altered chromosomes in Saccharomyces cerevisiae. Garber, P.M., Rine, J. Genetics (2002) [Pubmed]
  14. Identification and complete cDNA sequence of the missing Drosophila MCMs: DmMCM3, DmMCM6 and DmMCM7. Feger, G. Gene (1999) [Pubmed]
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