High impact information on DRS1

• Cold sensitivity and the assembly defect are recessive and cosegregate, defining a single essential gene that we designated DRS1 (deficiency of ribosomal subunits) [1].
• The wild-type DRS1 gene was cloned by complementation of the cold-sensitive phenotype of drs1 [1].
• DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae [2].
• The open reading frame of DRS1 corresponds to a protein of Mr = 57,061, in good agreement with the previously determined molecular weight of the purified enzyme [3].
• The nucleotide sequence of that cDNA, corresponding to the DRS1 gene, was determined [3].

Biological context of DRS1

• To identify new gene products that participate in ribosome biogenesis, we carried out a screen for mutations that result in lethality in combination with mutations in DRS1, a Saccharomyces cerevisiae nucleolar DEAD-box protein required for synthesis of 60S ribosomal subunits [4].

Other interactions of DRS1

• Thus Nop7p, like Drs1p, is required for essential steps leading to synthesis of 60S ribosomal subunits [4].

References