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Gene Review

RSA1  -  Rsa1p

Saccharomyces cerevisiae S288c

Synonyms: Ribosome assembly 1 protein, YPL193W
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Disease relevance of RSA1


High impact information on RSA1

  • The translational defect suggests eIF5B stabilizes Met-tRNA(i)(Met) binding and that GTP hydrolysis by eIF5B is a checkpoint monitoring 80S ribosome assembly in the final step of translation initiation [4].
  • Thus, all major posttranscriptional activities in ribosome synthesis, pre-rRNA processing, pre-rRNA modification, and ribosome assembly are dependent on fibrillarin [5].
  • The nop1.4 and nop1.7 alleles result in the synthesis of cytoplasmic 60S ribosomal subunits with strongly aberrant mobilities on sucrose gradients even at the permissive temperature, owing to the impairment of a late step in ribosome assembly [5].
  • Eukaryotic ribosomal proteins are required for production of stable ribosome assembly intermediates and mature ribosomes, but more specific roles for these proteins in biogenesis of ribosomes are not known [6].
  • These mutations not only reveal a more precise function for rpS14 in ribosome biogenesis but also uncover a role in ribosome assembly for the extended tails found in many ribosomal proteins [6].

Biological context of RSA1


Anatomical context of RSA1


Associations of RSA1 with chemical compounds


Physical interactions of RSA1

  • Contributions of the highly conserved K270 and its neighboring K271 in the C-terminal region of the yeast ribosomal protein L1 to 5S rRNA binding and ribosome assembly were examined by in vivo and in vitro studies on the consequences of 14 substitution mutations [16].

Regulatory relationships of RSA1

  • The rsa1 null allele synthetically enhances the mild growth defect of weak dbp6 alleles and confers synthetic lethality when combined with stronger dbp6 alleles [7].
  • Production of ribosomal protein S14 in Saccharomyces cerevisiae is coordinated with the rate of ribosome assembly by a feedback mechanism that represses expression of RPS14B [17].

Other interactions of RSA1

  • Synthetic lethality with conditional dbp6 alleles identifies rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits [7].
  • Interestingly, free 60S ribosomal subunits of a rsa1 null mutant strain that was grown for two generations at 37 degrees C are practically devoid of the 60S-ribosomal-subunit protein Qsr1p/Rpl10p, which is required for joining of 60S and 40S subunits (D. P. Eisinger, F. A. Dick, and B. L. Trumpower, Mol. Cell. Biol. 17:5136-5145, 1997) [7].
  • Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast [18].
  • Depletion of yeast ribosomal proteins L16 or rp59 disrupts ribosome assembly [19].
  • In L29 the corresponding Arg 25----Lys substitution within the nuclear localizing sequence distal to the N-terminus was without effect, as evidence by normal rates of ribosome assembly and cell growth [20].


  1. The m-AAA protease defective in hereditary spastic paraplegia controls ribosome assembly in mitochondria. Nolden, M., Ehses, S., Koppen, M., Bernacchia, A., Rugarli, E.I., Langer, T. Cell (2005) [Pubmed]
  2. Genome-wide analysis of mRNA stability using transcription inhibitors and microarrays reveals posttranscriptional control of ribosome biogenesis factors. Grigull, J., Mnaimneh, S., Pootoolal, J., Robinson, M.D., Hughes, T.R. Mol. Cell. Biol. (2004) [Pubmed]
  3. Structural and functional analyses of a yeast mitochondrial ribosomal protein homologous to ribosomal protein S15 of Escherichia coli. Dang, H., Ellis, S.R. Nucleic Acids Res. (1990) [Pubmed]
  4. Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity. Shin, B.S., Maag, D., Roll-Mecak, A., Arefin, M.S., Burley, S.K., Lorsch, J.R., Dever, T.E. Cell (2002) [Pubmed]
  5. Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. Tollervey, D., Lehtonen, H., Jansen, R., Kern, H., Hurt, E.C. Cell (1993) [Pubmed]
  6. The carboxy-terminal extension of yeast ribosomal protein S14 is necessary for maturation of 43S preribosomes. Jakovljevic, J., de Mayolo, P.A., Miles, T.D., Nguyen, T.M., Léger-Silvestre, I., Gas, N., Woolford, J.L. Mol. Cell (2004) [Pubmed]
  7. Synthetic lethality with conditional dbp6 alleles identifies rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits. Kressler, D., Doère, M., Rojo, M., Linder, P. Mol. Cell. Biol. (1999) [Pubmed]
  8. Inactivation of the RRB1-Pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. Killian, A., Le Meur, N., Sesboüé, R., Bourguignon, J., Bougeard, G., Gautherot, J., Bastard, C., Frébourg, T., Flaman, J.M. Oncogene (2004) [Pubmed]
  9. Modification of yeast ribosomal proteins. Phosphorylation. Kruiswijk, T., de Hey, J.T., Planta, R.J. Biochem. J. (1978) [Pubmed]
  10. Ribosomal protein L3 is involved in replication or maintenance of the killer double-stranded RNA genome of Saccharomyces cerevisiae. Wickner, R.B., Ridley, S.P., Fried, H.M., Ball, S.G. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  11. Protein substitution in chloroplast ribosome evolution. A eukaryotic cytosolic protein has replaced its organelle homologue (L23) in spinach. Bubunenko, M.G., Schmidt, J., Subramanian, A.R. J. Mol. Biol. (1994) [Pubmed]
  12. Assembly of the QM protein onto the 60S ribosomal subunit occurs in the cytoplasm. Nguyen, Y.H., Mills, A.A., Stanbridge, E.J. J. Cell. Biochem. (1998) [Pubmed]
  13. The p21-Activated Protein Kinase Inhibitor Skb15 and Its Budding Yeast Homologue Are 60S Ribosome Assembly Factors. Saveanu, C., Rousselle, J.C., Lenormand, P., Namane, A., Jacquier, A., Fromont-Racine, M. Mol. Cell. Biol. (2007) [Pubmed]
  14. Implications of a functional large ribosomal RNA with only three modified nucleotides. Sirum-Connolly, K., Peltier, J.M., Crain, P.F., McCloskey, J.A., Mason, T.L. Biochimie (1995) [Pubmed]
  15. Identification of an overexpressed yeast gene which prevents aminoglycoside toxicity. Prezant, T.R., Chaltraw, W.E., Fischel-Ghodsian, N. Microbiology (Reading, Engl.) (1996) [Pubmed]
  16. Involvement of lysine 270 and lysine 271 of yeast 5S rRNA binding protein in RNA binding and ribosome assembly. Yeh, L.C., Deshmukh, M., Woolford, J.L., Lee, J.C. Biochim. Biophys. Acta (1996) [Pubmed]
  17. Ribosomal protein S14 of Saccharomyces cerevisiae regulates its expression by binding to RPS14B pre-mRNA and to 18S rRNA. Fewell, S.W., Woolford, J.L. Mol. Cell. Biol. (1999) [Pubmed]
  18. Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast. Caesar, S., Greiner, M., Schlenstedt, G. Mol. Cell. Biol. (2006) [Pubmed]
  19. Depletion of yeast ribosomal proteins L16 or rp59 disrupts ribosome assembly. Moritz, M., Paulovich, A.G., Tsay, Y.F., Woolford, J.L. J. Cell Biol. (1990) [Pubmed]
  20. Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29. Underwood, M.R., Fried, H.M. EMBO J. (1990) [Pubmed]
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