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Gene Review

HTB1  -  histone H2B

Saccharomyces cerevisiae S288c

Synonyms: H2B1, Histone H2B.1, SPT12, Suppressor of Ty protein 12, YD9934.09C, ...
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High impact information on HTB1

  • First, the transcriptional defects in strains lacking these SNF genes are suppressed by a deletion of one of the two sets of genes encoding histones H2A and H2B, (hta1-htb1) delta [1].
  • We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations that inhibit adjacent gene expression [2].
  • We have identified a sequence element, designated the distal downstream element (DDE), that influences both the 3'-end cleavage site selection and the cell cycle regulation of the neo-HTB1 mRNA [3].
  • Mutations in the DDE, which is located approximately 110 nucleotides downstream of the HTB1 gene, lead to a delay in the accumulation of the neo-HTB1 mRNA in the S phase and a lack of mRNA turnover in the G(2) phase [3].
  • In Saccharomyces cerevisiae, fusion of the 3' untranslated region and downstream sequences of the yeast histone gene HTB1 to a neomycin phosphotransferase open reading frame is sufficient to confer cell cycle regulation on the resulting chimera gene (neo-HTB1) [3].

Biological context of HTB1

  • Here we show that a different mechanism of dosage compensation, at the level of gene copy number, can occur when HTA1-HTB1 is deleted [4].
  • The cell cycle regulation sequence is responsible for the periodic accumulation and hydroxyurea sensitivity of the histone HTA1-HTB1 message [5].
  • The HTA1-HTB1 locus causes suppression either when present on a high-copy-number plasmid or when mutant [2].

Associations of HTB1 with chemical compounds

  • This altered insertion pattern does not appear to be due to a bias caused by selecting canavanine resistant isolates in the different HTA1-HTB1 backgrounds [6].
  • The 3' end of the HTB1 gene containing a 17-amino-acid coding sequence and entire noncoding sequence was fused to the bacterial neomycin phosphotransferase II gene (neo) under control of the GAL1 promoter [7].

Regulatory relationships of HTB1

  • The level of the CDC14 transcript appears to be weakly cell cycle-regulated and has a periodicity which lags approximately 15 min behind histone HTB1 mRNA accumulation levels [5].

Other interactions of HTB1

  • Previous experiments demonstrated that mutations at one histone locus, HTA1-HTB1, do cause lethality when in conjunction with mutations in the SPT10 gene [8].
  • As yeast cells entered a synchronous cell cycle following release from alpha-factor arrest, the level of GAL1-promoter-controlled neo-HTB1 message increased approximately 12-fold during S phase and dropped to basal level when the cells left S phase [7].


  1. Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure. Hirschhorn, J.N., Brown, S.A., Clark, C.D., Winston, F. Genes Dev. (1992) [Pubmed]
  2. Changes in histone gene dosage alter transcription in yeast. Clark-Adams, C.D., Norris, D., Osley, M.A., Fassler, J.S., Winston, F. Genes Dev. (1988) [Pubmed]
  3. A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation. Campbell, S.G., Li Del Olmo, M., Beglan, P., Bond, U. Mol. Cell. Biol. (2002) [Pubmed]
  4. Amplification of histone genes by circular chromosome formation in Saccharomyces cerevisiae. Libuda, D.E., Winston, F. Nature (2006) [Pubmed]
  5. CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle. Wan, J., Xu, H., Grunstein, M. J. Biol. Chem. (1992) [Pubmed]
  6. Influences of histone stoichiometry on the target site preference of retrotransposons Ty1 and Ty2 in Saccharomyces cerevisiae. Rinckel, L.A., Garfinkel, D.J. Genetics (1996) [Pubmed]
  7. Coding and noncoding sequences at the 3' end of yeast histone H2B mRNA confer cell cycle regulation. Xu, H.X., Johnson, L., Grunstein, M. Mol. Cell. Biol. (1990) [Pubmed]
  8. SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. Dollard, C., Ricupero-Hovasse, S.L., Natsoulis, G., Boeke, J.D., Winston, F. Mol. Cell. Biol. (1994) [Pubmed]
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